Skip to main content
SpringerLink
Log in

Colletotrichum litangense sp. nov., Isolated as an Endophyte of Hippuris vulgaris, an Aquatic Plant in Sichuan, China

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

An unknown endophytic fungus was isolated from the aquatic plant Hippuris vulgaris in Litang county, Sichuan province, China. Phylogenetic analyses inferred from combined ITS, Sod2, Apn2, and TUB2 sequences revealed that the endophyte is a new species belonging to the Colletotrichum graminicola species complex. Morphological characteristics showed that Colletotrichum litangense is characterized by its falcate, lunate to sublunate conidia, and ellipsoidal, ovoid, or lobed appressoria. Pathogenicity tests on several fruits showed that C. litangense could induce anthracnose lesions. As a result of the phylogenetic, morphological, and pathogenicity analyses, we proposed the name Colletotrichum litangense for the new species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Data Availability

The relevant data are uploaded to the public database, and are available.

Code Availability

Not applicable.

References

  1. Eriksson OE, Baral HO, Currah RS, Hansen K, Kurtzman CP, Rambold G, Laessoe T (2003) Outline of ascomycota—2003. Myconet 9:1–189

    Google Scholar 

  2. Photita W, Taylor P, Ford R, Hyde K, Lumyong S (2005) Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand. Fungal Divers 18:117–133

    Google Scholar 

  3. Promputtha I, Lumyong S, Dhanasekaran V, Mckenzie E, Hyde KD, Jeewon R (2007) A phylogenetic evaluation of whether endophytes become saprotrophs at host senescence. Microb Ecol 53(4):579–590. https://doi.org/10.1007/s00248-006-9117-x

    Article  PubMed  Google Scholar 

  4. Diao YZ, Zhang C, Liu F, Wang WZ, Liu XL (2017) Colletotrichum species causing anthracnose disease of chili in China. Persoonia 38:20–37. https://doi.org/10.3767/003158517X692788

    Article  PubMed  Google Scholar 

  5. Zhang W, Damm U, Crous PW, Groenewald JZ, Li Y (2020) Anthracnose disease of carpetgrass (Axonopus compressus) caused by Colletotrichum hainanense sp. nov. Plant Dis 104(6):1–29. https://doi.org/10.1094/PDIS-10-19-2183-RE

  6. Dean R, Kan J, Pretorius ZA, Kim E, Hammond-Kosack PAD, Spanu PD et al (2012) The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol 13(7):414–430. https://doi.org/10.1111/j.1364-3703.2012.00822.x

    Article  PubMed  PubMed Central  Google Scholar 

  7. Arx JAV (1957) Die Arten der Gattung Colletotrichum Cda. Phytopathol Z 29:413–468

  8. Sutton BC (1992) The genus Glomerella and its anamorph Colletotrichum. CAB International, Wallingford, p 388

    Google Scholar 

  9. Cai L, Hyde KD, Taylor P, Weir BS, Liu ZY (2009) A polyphasic approach for studying Colletotrichum. Fungal Divers 39(2):183–204

    Google Scholar 

  10. Cannon PF, Damm U, Johnston PR, Weir BS (2012) Colletotrichum-current status and future directions. Stud Mycol 73(1):181–213. https://doi.org/10.3114/sim0014

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Crouch JA, Beirn L (2009) Anthracnose of cereals and grasses. Fungal Divers 39:19–44

    Google Scholar 

  12. Crouch JA, Clarke BB, White JF, Hillman BI (2009) Systematic analysis of the falcate-spored graminicolous Colletotrichum and a description of six new species from warm-season grasses. Mycologia 101(5):717–732. https://doi.org/10.3852/08-230

    Article  PubMed  Google Scholar 

  13. Sutton BC (1980) The coelomycetes: fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew, UK

    Google Scholar 

  14. Crouch JA (2004) Colletotrichum caudatum s.l. is a species complex. IMA Fungus 5(1):17–30. https://doi.org/10.5598/imafungus.2014.05.01.03

  15. Tao G, Liu ZY, Liu F, Gao YH, Cai L (2013) Endophytic Colletotrichum species from Bletilla ochracea (Orchidaceae), with descriptions of seven new species. Fungal Divers 61(1):139–164. https://doi.org/10.1007/s13225-013-0254-5

    Article  Google Scholar 

  16. Jayawardena RS, Hyde KD, Damm U, Cai L, Yan JY (2016) Notes on currently accepted species of Colletotrichum. Mycosphere 7(8):1192–1260. https://doi.org/10.5943/mycosphere/si/2c/9

    Article  Google Scholar 

  17. Wang QT, Liu F, Hou CL, Cai L (2021) Species of Colletotrichum on bamboos from China. Mycologia 113(2):1–9. https://doi.org/10.1080/00275514.2020.1837567

    Article  Google Scholar 

  18. Zheng H, Yu Z, Xu J, Castañeda-Ruiz RF, Qiao M (2020) Ramichloridium endophyticum sp. nov., a novel species of endophytic fungus from Potamogeton pectinatus. Int J Syst Evol Microbiol 70(5):3433–3439. https://doi.org/10.1099/ijsem.0.004190

  19. Rayner RW (1970) A mycological colour chart. Kew Commonw Mycolog Inst 64(1):230

    Google Scholar 

  20. White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Academic Press Inc, New York, pp 315–322

  21. Crouch JA, Tredway LP, Clarke BB, Hillman BI (2010) Phylogenetic and population genetic divergence correspond with habitat for the pathogen Colletotrichum cereale and allied taxa across diverse grass communities. Mol Ecol 18(1):123–135. https://doi.org/10.1111/j.1365-294X.2008.04008.x

    Article  CAS  Google Scholar 

  22. Glass NL, Donaldson GC, Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61(4):1323–1330. https://doi.org/10.1002/bit.260460112

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus fusarium are nonorthologous. Mol Phylogenet Evol 7(1):103–116. https://doi.org/10.1006/mpev.1996.0376

    Article  CAS  PubMed  Google Scholar 

  24. Thompson JD (1997) The Clustal_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  25. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  26. Sudhir KS, Glen, Koichiro T (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874. https://doi.org/10.1093/molbev/msw054

  27. Huelsenbeck J (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17(8):754–755. https://doi.org/10.1093/bioinformatics/17.8.754

    Article  CAS  PubMed  Google Scholar 

  28. Rambaut A (2012) FigTree v1.4.2. Program distributed by the author, http://tree.bio.ed.ac.uk/software/figtree/2012

  29. Chung PC, Wu HY, Wang YW, Ariyawansa HA, Chung CL (2020) Diversity and pathogenicity of Colletotrichum species causing strawberry anthracnose in Taiwan and description of a new species Colletotrichum miaoliense sp. nov. Sci Rep 10(1):14664. https://doi.org/10.1038/s41598-020-70878-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Montri P, Taylor PWJ, Mongkolporn O (2009) Pathotypes of Colletotrichum capsici, the causal agent of chili anthracnose, in Thailand. Plant Dis 93(1):17–20. https://doi.org/10.1094/PDIS-93-1-0017

    Article  CAS  PubMed  Google Scholar 

  31. Sato T, Moriwaki J, Uzuhashi S, Degawa Y, Ono T, Nishimura K (2012) Molecular phylogenetic analyses and morphological re-examination of strains belonging to three rare Colletotrichum species in Japan. Microbiol Cult Collect 28:121–134

    Google Scholar 

  32. Suryanarayanan TS, Murali TS, Venkatesan G (2002) Occurrence and distribution of fungal endophytes in tropical forests across a rainfall gradient. Can J Bot 80(8):818–826. https://doi.org/10.1139/b02-069

    Article  Google Scholar 

  33. Marcelino J, Giordano R, Gouli S, Gouli V, Parker BL, Skinner M, TeBeest D, Cesnik R (2008) Colletotrichum acutatum var. fioriniae (teleomorph: Glomerella acutata var. fioriniae var. nov.) infection of a scale insect. Mycologia 100(3):353–374. https://doi.org/10.3852/07-174R

  34. Manamgoda DS, Udayanga D, Lei C, Chukeatirote E (2013) Endophytic Colletotrichum from tropical grasses with a new species C. endophytica. Fungal Divers 61(1):107–115. https://doi.org/10.1007/s13225-013-0256-3

  35. Vieira W, Michereff SJ, de Morais Jr MA, Hyde KD, Cmara MPS (2014) Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal Divers 67(1):1–22. https://doi.org/10.1007/s13225-014-0293-6

    Article  Google Scholar 

  36. Chen KL, Kirschner R (2017) Fungi from leaves of lotus (Nelumbo nucifera). Mycol Prog 17:275–293. https://doi.org/10.1007/s11557-017-1324-y

    Article  Google Scholar 

  37. Sakayaroj J, Preedanon S, Supaphon O, Jones E, Phongpaichit S (2010) Phylogenetic diversity of endophyte assemblages associated with the tropical seagrass Enhalus acoroides in Thailand. Fungal Divers 42(1):27–45. https://doi.org/10.1007/s13225-009-0013-9

    Article  Google Scholar 

  38. Gasich EL, Titova YA, Berestetsky AO (1999) The herbaceous wild plants mycobiota of the Valaam island. Mikol Fitopatol 33(6):392–401

    Google Scholar 

  39. Anonymous (1979) List of plant diseases in Taiwan. 404

  40. Mathur RS (1979) The Coelomycetes of India. Bishen Singh Mahendra Pal Singh, Delhi

  41. Xue L, Zhang Y, Duan T, White JF, Li C (2020) Characterization and pathogenicity of Colletotrichum species on Philodendron tatei cv. Congo in Gansu Province, China. Plant Dis 104(10):1–35. https://doi.org/10.1094/PDIS-09-19-1952-RE

Download references

Acknowledgements

We are grateful to two reviewers for critically reviewing the manuscript and for providing helpful suggestions to improve this paper.

Funding

This work was financed by the National Natural Science Foundation Program of PR China (31760012, 31970013).

Author information

Authors and Affiliations

  1. Laboratory for Conservation and Utilization of Bio-resources, Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Jie Li, Hua Zheng, Ze-fen Yu & Min Qiao

Authors
  1. Jie Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hua Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ze-fen Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Min Qiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to the study conception and design. HZ and JL wrote the manuscript. JL conducted the experiments. ZY and MQ revised the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Min Qiao.

Ethics declarations

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical Approval

Not applicable.

Consent to Participate

Not applicable.

Consent for Publication

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The GenBank accession numbers of Colletotrichum litangense: ITS MW722955; TUB2 MW776748; Sod2 MW776746; Apn2 MW776747.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, J., Zheng, H., Yu, Zf. et al. Colletotrichum litangense sp. nov., Isolated as an Endophyte of Hippuris vulgaris, an Aquatic Plant in Sichuan, China. Curr Microbiol 79, 161 (2022). https://doi.org/10.1007/s00284-022-02846-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00284-022-02846-0

Search

Navigation