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Oceanospirillum sediminis sp. nov., Isolated From Coastal Sediment in the Yellow Sea

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Abstract

A novel Gram-negative, motile, aerobic, spiral-shaped bacterium designated D5T, was isolated from a coastal sediment collected in the Yellow Sea. Optimal growth occurred at 30 °C, pH 7.0–8.0 and in the presence of 1–3% (w/v) NaCl. Strain D5T contained ubiquinone 8 (Q-8) as the predominant respiratory quinone. The major fatty acids (> 10%) were C16:0, C16:1 ω7c/C16:1 ω6c and C18:1w7c/C18:1w6c. The main polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The draft genome is 5.6 Mb in length, and DNA G + C content is 47.2 mol%. 16S rRNA gene sequences showed that strain D5T is most closely related to Oceanospirillum beijerinckii NBRC 15445T (97.8%, sequence similarity). However, the digital DNA-DNA hybridization (dDDH) value and average nucleotide identity (ANI) between strain D5T and O. beijerinckii is only 27.8% and 77.1%. Phylogenetic trees based on 16S rRNA gene sequences and whole genomes all indicated that strain D5T formed a separate branch in the genus Oceanospirillum. Combined results of the polyphasic analyses suggested that strain D5T represents a novel species in the genus Oceanospirillum, for which the name Oceanospirillum sediminis sp. nov. is proposed. The type strain is D5T (= MCCC 1K06061T = KCTC 62987T).

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Acknowledgements

We sincerely thank Prof. Aharon Oren in Hebrew University of Jerusalem for advice on nomenclature. This work was supported by Shandong Provincial Natural Science Foundation (Grant Nos. 2020KJK006, ZR2020MH297).

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  1. Huilin Zhao and Yue Tian have contributed equally to the paper.

Authors and Affiliations

  1. School of Basic Medical Sciences, Binzhou Medical University, Yantai, China

    Huilin Zhao, Yue Tian, Qianwen Wu, Si Chen, Jiangfan Shan, Boqing Li & Xiaofei Ji

  2. College of Marine Science, Shandong University, Weihai, China

    Xunke Sun & Zongjun Du

  3. School of Clinical Medicine, Binzhou Medical University, Yantai, China

    Yu Zhong, Xinyu Chen, Xiaoxue Gao, Shengnan Liu & Ruochen Wang

  4. State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China

    Zongjun Du

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  1. Huilin Zhao
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Contributions

Conceive and design the experiments: XJ, HZ and BL; Perform the experiments: XJ, HZ, YT, XS, QW, SC, JS, YZ, XC, XG, SL, RW and ZD; Writing the paper: XJ and HZ; Revising the paper: XJ and BL; All authors approved the final manuscript.

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Correspondence to Boqing Li or Xiaofei Ji.

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Zhao, H., Tian, Y., Sun, X. et al. Oceanospirillum sediminis sp. nov., Isolated From Coastal Sediment in the Yellow Sea. Curr Microbiol 79, 124 (2022). https://doi.org/10.1007/s00284-022-02816-6

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