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Janibacter endophyticus sp. nov., an Endophytic Actinobacterium Isolated from the Root of Paris polyphylla Smith var. Yunnanensis

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Abstract

A novel endophytic actinobacterium, designated as strain YIM B02568T, was isolated from the root of Paris polyphylla Smith var. Yunnanensis obtained from Yunnan Province, southwest China. Strain YIM B02568T was characterized using a polyphasic approach. Phylogenetic analysis indicated that this isolate belonged to the genus Janibacter. The 16S rRNA gene sequence similarity values of strain YIM B02568T to the type strains of members of this genus ranged from 95.8 to 98.6%. However, overall genome relatedness indices were significantly lower than the widely accepted species-defined threshold. The cell wall of strain YIM B02568T contained meso-diaminopimelic acid. The major menaquinone was MK-8(H4). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylinositol. The major cellular fatty acids were comprised of iso-C16:0 and C18:1 ω9c. The DNA G + C content was 71.6 mol%. Based on the data from the polyphasic studies, we propose that strain YIM B02568T represents a novel species within the genus Janibacter, Janibacter endophyticus sp. nov. The type strain is YIM B02568T (= JCM 34639T = CGMCC 1.18658T).

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Abbreviations

NA:

Nutrient agar

ANI:

Average nucleotide identity

AAI:

Average amino acid identity

dDDH:

Digital DNA–DNA hybridization

GCM:

Global Catalogue of Microorganisms

MK:

Menaquinone

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31970122 and 32060003), the Natural Science Foundation of Yunnan Province (202001BB050040), the Major Science and Technology Projects of Yunnan Province (Digitalization, development, and application of biotic resource 202002AA100007).

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  1. Zhen Zhang and En-Min Zhou have contributed equally to this work.

Authors and Affiliations

  1. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    Zhen Zhang, Cong-Jian Li, Xing-Wang Jiang, Rui-Feng Mao, Jing-Ran Liu, Xiao-Yang Zhi & Ling-Ling Yang

  2. International Joint Research Center for Karstology, School of Earth Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    En-Min Zhou

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Contributions

ZZ and E-MZ carried out the data analysis and prepared the manuscript. ZZ, C-JL, X-WJ, R-FM, and J-RL performed the polyphasic taxonomy. X-WJ and L-LY prepared the experiments and isolated the novel strain. L-LY and X-YZ designed the experiment and directed the classification. L-LY takes full responsibility for the final submission. All the authors reviewed and approved the final version of the paper.

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Correspondence to Ling-Ling Yang.

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Zhang, Z., Zhou, EM., Li, CJ. et al. Janibacter endophyticus sp. nov., an Endophytic Actinobacterium Isolated from the Root of Paris polyphylla Smith var. Yunnanensis. Curr Microbiol 79, 52 (2022). https://doi.org/10.1007/s00284-021-02745-w

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