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Buttiauxella massiliensis sp. nov., Isolated from a Human Bone Infection

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Abstract

Strain Marseille-P9829 was isolated from a bone sample collected from an open right fibula fracture from a 46-years old patient. Strain Marseille-P9829 (= CSUR P9829 = DSM 110695) was a Gram-negative, non-spore-forming and non-motile bacterium. This strain had a positive catalase activity but was oxidase-negative. The major fatty acids methyl esters were hexadecanoic acid (45.6%) and 9-hexadecenoic acid (28.4%). Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry analysis suggested that this strain belongs to the species Buttiauxella gaviniae. Since there were few reports of clinical infections with this species in humans, whole genome sequencing was performed and a polyphasic taxono-genomic approach was followed in order to verify the classification of strain Marseille-P9829. The 16S rRNA gene sequence BLAST against the NCBI database yielded the highest similarity of 99.8% with Buttiauxella agrestis, suggesting that strain Marseille-P9829 belongs to this species. However, genomic comparison by digital DNA–DNA hybridization showed that values between strain Marseille-P9829 and other validly published Buttiauxella species were all lower than 70%. Furthermore, all average nucleotide identities were lower than 95–96%. Therefore, these results confirmed that strain Marseille-P9829 belonged to a new Buttiauxella species for which we propose the name Buttiauxella massiliensis sp. nov., with strain Marseille-P9829 as type strain.

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Data Availability

The 16S rRNA gene sequence and genome sequence of strain Marseille-P9829 are deposited in GenBank under accession numbers MZ061590 and CABVOV000000000, respectively.

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Acknowledgements

We thank Takashi Irie, Kyoko Imai, Shigeki Matsubara, Taku Sakazume, Yusuke Ominami, Hisada Akiko and the Hitachi team of Japan (Hitachi High-Technologies Corporation, Science & Medical Systems Business Group 24-14, Nishi-shimbashi 1-chome, Minato-ku, Tokyo 105-8717 Japan) for the collaborative study conducted together with IHU Méditerranée Infection, and for the installation of a SU5000 microscope at IHU Méditerranée Infection facility.

Funding

This work was supported by Méditerranée Infection and the National Research Agency under the program “Investissements d’avenir”, reference ANR-10-IAHU-03 and Region Provence Alpes Côte d’Azur and European funding FEDER IHUBIOTK.

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Authors and Affiliations

  1. Aix Marseille Université, Institut de Recherche pour le Développement (IRD), Service de Santé des Armées, AP-HM, UMR Vecteurs Infections Tropicales et Méditerranéennes (VITROME), Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France

    Rita Zgheib, Hussein Anani, Florence Fenollar & Pierre-Edouard Fournier

  2. Institut Méditerranée-Infection, Marseille, France

    Rita Zgheib, Issam Hasni, Babacar Mbaye, Hussein Anani, Gabriel Haddad, Nicholas Armstrong, Céline Chartier, Aurélia Caputo, Didier Raoult, Florence Fenollar & Pierre-Edouard Fournier

  3. Aix-Marseille Université, Institut de Recherche pour le Développement (IRD), UMR Microbes Evolution Phylogeny and Infections (MEPHI), Institut Hospitalo-Universitaire Méditerranée-Infection, Marseille, France

    Issam Hasni, Babacar Mbaye, Gabriel Haddad, Aurélia Caputo & Didier Raoult

  4. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia

    Didier Raoult

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  1. Rita Zgheib
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Contributions

RZ, HA and IH performed the phylogenomic analysis and drafted the manuscript. Phenotypic and biochemical tests were performed by BM. GH performed scanning electron microscopy. NA and CC performed the fatty acid analyses. AC handled data deposition in GenBank. DR, FF and PEF conceived, designed and supervised the study. All authors participated in writing the final version of the manuscript.

Corresponding author

Correspondence to Pierre-Edouard Fournier.

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Conflict of interest

From March 2018 to March 2021, Pr. Raoult was scientific advisor for the Hitachi High-Tech Corporation for the application of electron microscopy to routine clinical microbiology. The authors declare no other conflict of interest.

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Zgheib, R., Hasni, I., Mbaye, B. et al. Buttiauxella massiliensis sp. nov., Isolated from a Human Bone Infection. Curr Microbiol 79, 41 (2022). https://doi.org/10.1007/s00284-021-02714-3

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