Abstract
The purpose of this study was to discuss the effects of an extract from the culture medium of Pseudomonas aeruginosa (P. aeruginosa) 2016NX1 (chloroform extract of P. aeruginosa, CEPA) and its purified product 1-hydroxyphenazine on RAW264.7 cell inflammation. Cell viability was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method. TNF-α production was determined by an ELISA method. The effects of CEPA and its purified product 1-hydroxyphenazine on cell morphology were investigated using an inverted microscope. Quantitative real-time PCR was performed to determine mRNA expression levels. CEPA and 1-hydroxyphenazine had no obvious toxicity to cells when their concentrations were no more than 20 μg ml−1 and 5 μg ml−1, respectively. Both CEPA and 1-hydroxyphenazine suppressed the secretion of TNF-α and significantly reduced the mRNA expression levels of TNF-α, IL-1β, and IL-6. Both CEPA and 1-hydroxyphenazine inhibited M1 cell polarization after lipopolysaccharide (LPS) stimulation. The results in this article lay a good foundation for the biopharmaceutical applications of CEPA and 1-hydroxyphenazine in the future. CEPA and 1-hydroxyphenazine had certain anti-inflammatory activity, and inhibited LPS-induced RAW264.7 cell inflammation. Our findings suggest that CEPA and 1-hydroxyphenazine are potential chemicals with anti-inflammatory activity.
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Acknowledgements
This research was funded by the National Natural Science Foundation of China (No. 31860010), the Natural Science Foundation of Guangxi Zhuang autonomous region (No. 2017JJA130300y and 2018GXNSFAA138008), and Science and Technology Program of Guangzhou, China (No. 201804010155).
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JX wrote the main part of the paper and performed the experiments. AAT, TL, DG, and WL wrote the less part of the paper. CS and ZL conceived the experiments and proofread the paper. All the authors read and approved the manuscript.
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Xiao, J., Thwe, A.A., Liu, T. et al. Anti-Inflammatory Effects of an Extract from Pseudomonas aeruginosa and Its Purified Product 1-Hydroxyphenazine on RAW264.7 Cells. Curr Microbiol 78, 2762–2773 (2021). https://doi.org/10.1007/s00284-021-02544-3
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DOI: https://doi.org/10.1007/s00284-021-02544-3