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Effects of Amino Acids and Overexpression of dapA Gene on the Production of ε-Poly-L-lysine by Streptomyces diastatochromogenes Strains

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Abstract

In this study, the strain Streptomyces diastatochromogenes 6#-7, which efficiently synthesizes ε-Poly-L-lysine, was studied and the effects of 18 amino acids and overexpression of dapA gene on the fermentation efficiency of ε-PL by S. diastatochromogenes were investigated. It was shown that L-proline, L-lysine, L-isoleucine, and L-threonine could promote the production of ε-PL. Moreover, the overexpression of the dihydrodipicolinate synthase gene (dapA) helped improve the fermentation performance of S. diastatochromogenes. The maximum ε-PL yield of the overexpressing strain (S. diastatochromogenes 12#-2) increased by 17.5% compared with the original strain in 500 mL shake flask. When the fermentation was conducted in a 5-L fermenter, the fermentation duration was extended by 48 h, and ε-PL yield reached 30.54 g/L, which was a 19.8% increase compared to the original strain. The results of this study offered a promising approach to augment the production of ε-PL from Streptomyces, thus paving the way to reduce the cost of product ε-PL and enhance the fermentation efficiency of ε-PL production.

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Funding

This work was financially supported by the National Key R&D Program of China (Project NO. 2018YFD0400205), and the National Natural Fund General Program of China (Project NO. 31771952) and the Open Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology (No. SKLFNS-KF-201911).

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, PR China

    Wenchao Li, Junge Lv, Tianyu Dong, Xinying Li, Xiaona Li, Zhilei Tan & Shiru Jia

  2. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, PR China

    Wenchao Li, Junge Lv, Tianyu Dong, Xinying Li, Xiaona Li, Zhilei Tan & Shiru Jia

  3. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, PR China

    Zhilei Tan & Shiru Jia

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  1. Wenchao Li
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Li, W., Lv, J., Dong, T. et al. Effects of Amino Acids and Overexpression of dapA Gene on the Production of ε-Poly-L-lysine by Streptomyces diastatochromogenes Strains. Curr Microbiol 78, 2640–2647 (2021). https://doi.org/10.1007/s00284-021-02510-z

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