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Marinisubtilis pacificus gen. nov., sp. nov., a Member of the Family Microbacteriaceae Isolated From a Deep-Sea Seamount

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Abstract

A Gram-stain-positive, yellow, aerobic, slender rod-shaped bacterial strain, designated KN1116T, was isolated from a deep-sea seamount. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KN1116T was related to the genus Chryseoglobus and had highest 16S rRNA gene sequence identity with Chryseoglobus frigidaquae CW1T (98.5%). The predominant cellular fatty acids were anteiso-C15:0 and iso-C16:0. The quinone system for strain KN1116T comprised menaquinone MK-12, MK-11, MK-10 and MK-13. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, six unknown glycolipids, two unidentified phospholipids and one unknown polar lipid. The cell-wall peptidoglycan of strain KN1116T was of the type B1β, containing 2,4-diaminobutyric acid as the diamino acid. Genome sequencing revealed the strain KN1116T has a genome size of 2.7 Mbp and a G+C content of 69.4 mol%. Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain KN1116T represents a novel species of a novel genus of the family Microbacteriaceae, for which the name Marinisubtilis pacificus gen. nov., sp. nov. is proposed. The type strain of Marinisubtilis pacificus is KN1116T (=CGMCC 1.17143T =KCTC 49299T).

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Acknowledgements

We are grateful for Center for Ocean Mega-Science (Chinese Academy of Sciences). We thank Dr Peter Schumann (DSMZ) for the analysis of the peptidoglycan structure.

Funding

This study was supported by the National Natural Science Foundation of China (31670002) and the Science & Technology Basic Resources Investigation Program of China (2017FY100804) and the Senior User Project of RV KEXUE (KEXUE2019G09).

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Authors and Affiliations

  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Fang Qin, Wen-Xuan He & De-Chao Zhang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Fang Qin, Wen-Xuan He & De-Chao Zhang

  3. Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China

    De-Chao Zhang

Authors
  1. Fang Qin
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  2. Wen-Xuan He
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Contributions

All authors contributed to the study conception and design. ZDC designed this research, QF and HWX isolated the strain and performed the initial cultivation and strain deposition, QF performed strain characterization, the electron microscopic and chemotaxonomic analysis, HWX performed the genomic and phylogenetic analysis, and QF and HWX drafted the manuscript. ZDC supervised the study and contributed to text preparation and revised the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to De-Chao Zhang.

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Qin, F., He, WX. & Zhang, DC. Marinisubtilis pacificus gen. nov., sp. nov., a Member of the Family Microbacteriaceae Isolated From a Deep-Sea Seamount. Curr Microbiol 78, 2136–2142 (2021). https://doi.org/10.1007/s00284-021-02468-y

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  • DOI: https://doi.org/10.1007/s00284-021-02468-y

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