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Isolation and Identification of Pseudomonas sp. Strain DY-1 from Agricultural Soil and Its Degradation Effect on Prometryne

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Abstract

Prometryne is a widely used herbicide in China to control annual grasses and broadleaf weeds. However, the stability of prometryne makes it difficult to be degraded, which poses a threat to human health. This study presents a bacterial strain isolated from soil samples with a prometryne application history, designated strain DY-1. Strain DY-1, identified as Pseudomonas sp., is capable of utilizing prometryne as a sole carbon source for growth and degrading 100% of prometryne within 48 h from an initial concentration of 50 mg L−1. To further optimize the degradation of prometryne, the prometryne concentration, temperature, pH, and salt concentration were examined. The optimal conditions for degradation of prometryne by strain DY-1 were an initial prometryne concentration of 50 mg L−1, 30 °C, pH 7–8, and NaCl concentration of 200 mg L−1. The same strain also degraded other s-triazine herbicides, including simetryne, ametryne, desmetryne, and metribuzin, under the same conditions. The biodegradation pathway of prometryne was established by isolating sulfoxide prometryne as the first metabolite and by the identification of sulfone prometryne and 2-hydroxy prometryne by liquid chromatography-mass spectrometry (LC–MS/MS). The results illustrated that strain DY-1 achieved the removal of prometryne by gradually oxidizing and hydrolyzing the methylthio groups. A bioremediation trial with contaminated soil and pot experiments showed that after treating the prometryne-contaminated soil with strain DY-1, the content of prometryne was significantly reduced (P < 0.05). This study provides an efficient bacterial strain and approach that could be potentially useful for detoxification and bioremediation of prometryne analogs.

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Data Availability

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgments

Special acknowledgment is given to all the participants in this research and their relatives for their cordial guidance and material support during the laboratory work. The authors thank the National Key R & D Projects (2017YFD0201200) and Heilongjiang Provincial National Science Foundation (C2016025) for support, and Springer Nature Research Editing Service for grammar editing.

Funding

This research was funded by the National Key R & D Projects (Grant No. 2017YFD0201200) and Heilongjiang Provincial National Science Foundation (Grant No. C2016025).

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Authors and Affiliations

  1. College of Life Science, Northeast Agricultural University, Harbin, 150038, China

    Dong Liang, Ming-yue Ding, Chang-yixin Xiao, Ya-wen Shen, Yin-yue Wang, Hai-tao Li, Rong-mei Liu & Ji-guo Gao

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  1. Dong Liang
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  2. Ming-yue Ding
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Contributions

The manuscript was written through contributions of all authors. All the authors have given approval to the final version of the manuscript. DL, HL, and RL contributed to conceptualization; DL and MD were involved in methodology; YS and YW performed experiment; DL was involved in writing—original draft preparation; DL and CX were involved in writing—editing; JG contributed to funding acquisition; HL and RL supervised the study.

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Correspondence to Rong-mei Liu or Ji-guo Gao.

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Liang, D., Ding, My., Xiao, Cy. et al. Isolation and Identification of Pseudomonas sp. Strain DY-1 from Agricultural Soil and Its Degradation Effect on Prometryne. Curr Microbiol 78, 1871–1881 (2021). https://doi.org/10.1007/s00284-021-02433-9

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