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Short-Chain Poly(γ-diaminobutanoic acid), A Poly(amino acid) Produced by a Marine Bacteria Bacillus pumilus

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Abstract

One marine bacteria Bacillus pumilus was isolated using allura red as ε-poly-L-lysine (ε-PL) secretion indicator. But actually the product was identified as poly-γ-L-diaminobutanoic acid (γ-PAB) by ionization-time-of-flight mass spectrometry, not coproduced with ε-PL. The polymerization degree of γ-PAB was 4–22, namely short-chain γ-PAB, compared with that in S. celluloflavus, and it exhibited stronger inhibitory activities against yeasts than long-chain γ-PAB but weaker activities against bacteria. The fermentative behavior of B. pumilus was investigated, and the γ-PAB production was 38.6 mg/L in shake flask and was enhanced to 284.2 mg/L in 5-L bioreactor by a pH control strategy. Interestingly, the suitable pH for B. pumilus to produce γ-PAB was 4.8, different from 4.0 for current Streptomyces strains, which suggests a potential new metabolic mechanism in B. pumilus as a novel γ-PAB producer. No studies on short-chain γ-PAB production in bacteria have been reported previously and we considered that this is a new discovery in the field of homopolymer research.

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All data generated or analysed during this study are included in this published article.

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Acknowledgement

We appreciated Liang Wang for mass spectrometry analysis in the manuscript.

Funding

This work was financially supported by Shandong Natural Science Foundation (ZR2019BC044).

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Authors and Affiliations

  1. Marine College, Shandong University, Weihai, 264209, Shandong, China

    Shu Li, Yao Yao, Shengjie Hu & Junru Chen

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  1. Shu Li
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  2. Yao Yao
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  3. Shengjie Hu
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  4. Junru Chen
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Correspondence to Shu Li.

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Li, S., Yao, Y., Hu, S. et al. Short-Chain Poly(γ-diaminobutanoic acid), A Poly(amino acid) Produced by a Marine Bacteria Bacillus pumilus. Curr Microbiol 78, 1142–1149 (2021). https://doi.org/10.1007/s00284-021-02371-6

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  • DOI: https://doi.org/10.1007/s00284-021-02371-6

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