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Effects of Rhein-8-O-β-D-glucopyranoside on the Biofilm Formation of Streptococcus mutans

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Abstract

Dental caries is the most frequent biofilm-related human infectious disease in the oral cavity. Streptococcus mutans is one of the primary etiological agents of dental caries. The aim of our study was to investigate the effects of rhein-8-O-β-D-glucopyranoside (Rg) on the development of S. mutans biofilms. Growth curves were generated, and biofilm oxygen sensitivity was detected after Rg treatment. The expression levels of luxS, brpA, ffh, recA, nth, and smx were analyzed by real-time PCR. The trypan blue exclusion assay was used to measure the effect of Rg on monocyte viability. The results showed that Rg could significantly inhibit the growth of S. mutans and suppress the biofilm formation of S. mutans in a concentration-dependent manner. In Rg-treated biofilms, the expression levels of luxS, brpA, ffh, recA, nth, and smx were all decreased. Our results further showed that Rg was nontoxic, as Rg did not affect monocyte viability or lactate dehydrogenase activity in the exposed cells. These results suggested that Rg inhibited the biofilm formation of S. mutans, and the decrease in luxS, brpA, ffh, recA, nth, and smx expression might contribute to the antibacterial effects of Rg.

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Funding

The present study was supported by the Special scientific research plan project of Shaanxi provincial department of education (project number: 18JK0656), Key plan of Shaanxi provincial science and technology department (project number: 2017sf-111), and Youth scientific research fund project of Xi'an Medical College (project number: 2015QN13).

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Authors and Affiliations

  1. College of Stomatology, Xi’an Medical University, South 2nd Ring Road NO.168, Yan ta District, Xi’an, Shaanxi Province, China

    Yaochao Zhang, Yong Zhu, Yanping Zuo, Chengfang Tang, Fang Zhou, Xiaoming Cui & Lin Wang

Authors
  1. Yaochao Zhang
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  2. Yong Zhu
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  3. Yanping Zuo
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  4. Chengfang Tang
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  5. Fang Zhou
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  6. Xiaoming Cui
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  7. Lin Wang
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Contributions

YZ contributed to the conception of the study. YZ, YZ, and YZ performed the experiment. CT, FZ, XC, and LW performed the data analyses. YZ wrote the manuscript.

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Correspondence to Yaochao Zhang.

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Zhang, Y., Zhu, Y., Zuo, Y. et al. Effects of Rhein-8-O-β-D-glucopyranoside on the Biofilm Formation of Streptococcus mutans. Curr Microbiol 78, 323–328 (2021). https://doi.org/10.1007/s00284-020-02248-0

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  • DOI: https://doi.org/10.1007/s00284-020-02248-0

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