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Complete Genome Sequence of Lactobacillus hilgardii LMG 7934, Carrying the Gene Encoding for the Novel PII-Like Protein PotN

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Abstract

Lactic acid bacteria are widespread in various ecological niches with the excess of nutrients and have reduced capabilities to adapt to starvation. Among more than 280 Lactobacillus species known to the date, only five, including Lactobacillus hilgardii, carry in their genome the gene encoding for PII-like protein, one of the central regulators of cellular metabolism generally responding to energy- and carbon–nitrogen status in many free-living Bacteria, Archaea and in plant chloroplasts. In contrast to the classical PII encoding genes, in L. hilgardii genome the gene for PII homologue is located within the potABCD operon, encoding the ABC transporter for polyamines. Based on the unique genetic context and low sequence identity with genes of any other so-far characterized PII subfamilies, we termed this gene potN (Pot-protein, Nucleotide-binding). The second specific feature of L. hilgardii genome is that many genes encoding the proteins with similar function are present in two copies, while with low mutual identity. Thus, L. hilgardii LMG 7934 genome carries two genes of glutamine synthetase with 55% identity. One gene is located within classical glnRA operon with the gene of GlnR-like transcriptional regulator, while the second is monocistronic. Together with the relative large genome of L. hilgardii as compared to other Lactobacilli (2.771.862 bp vs ~ 2.2 Mbp in median), these data suggest significant re-arrangements of the genome and a wider range of adaptive capabilities of L. hilgardii in comparison to other bacteria of the genus Lactobacillus.

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Acknowledgements

This research was supported in the form of a grant from the President of the Russian Federation for state support to young Russian scientists—doctors of sciences (MD- 572.2020.4 for AK) and by infrastructural funding from DFG Cluster of Excellence EXC 2124 (Controlling Microbes to Fight Infections) at University Tübingen. This work has been performed in frames of Russian Government Program of Competitive Development of Kazan Federal University. The genome assembly and analysis was performed using the facilities of the Scientific and Educational Mathematical Center of the Volga Federal District (Project No. 075-02-2020-1478).

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    Darya E. Zhuravleva, Zalina I. Iskhakova, Georgii D. Ozhegov, Natalia E. Gogoleva, Dilyara R. Khusnutdinova, Elena I. Shagimardanova & Airat R. Kayumov

  2. Kazan Institute of Biochemistry and Biophysics, Kazan Science Centre, Russian Academy of Sciences, Kazan, Russia

    Natalia E. Gogoleva

  3. Eberhard Karls University Tübingen, Tübingen, Germany

    Karl Forchhammer

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  1. Darya E. Zhuravleva
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Conceptualization—AK and KF; methodology—GO, NG, DK, ES; investigation—DZ, ZI, GO; formal analysis—DZ, AK and KF; resources—AK and KF; visualization—GO; project administration—AK and KF; supervision—AK and KF; funding acquisition—AK and KF; writing—original draft preparation, review and editing—DZ, AK and KF.

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Correspondence to Airat R. Kayumov.

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Authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Zhuravleva, D.E., Iskhakova, Z.I., Ozhegov, G.D. et al. Complete Genome Sequence of Lactobacillus hilgardii LMG 7934, Carrying the Gene Encoding for the Novel PII-Like Protein PotN. Curr Microbiol 77, 3538–3545 (2020). https://doi.org/10.1007/s00284-020-02161-6

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