Abstract
A Gram-negative, aerobic, non-motile, rod-shaped, floc-forming, and non-spore-forming bacterium, designated as NLF-7-7T, was isolated from the biofilm of a sample collected from a livestock wastewater treatment plant in Nonsan, Republic of Korea. Strain NLF-7-7T, forms a visible floc and grows in the flocculated state. Cells of strain NLF-7-7T grew optimally at pH 6.5 and 30 °C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NLF-7-7T belonged to the family Comamonadaceae, and was most closely related to Comamonas badia DSM 17552T (95.8% similarity) and Comamonas nitrativorans 23310T (94.0% similarity). The phylogenetic and phenotypic data indicate strain NLF-7-7T is clearly distinguished from the Comamonas lineage. The major cellular fatty acids were C10:0 3OH, C16:0, and summed feature 3 (C16:1 ω6c/C16:1 ω7c). The respiratory quinone was Q-8. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unidentified aminolipid. The DNA G+C content of strain NLF-7-7 was 68.0 mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic properties, strain NLF-7-7T represents a novel species of the genus Comamonas, for which the name Comamonas flocculans sp. nov. is proposed. The type strain is C. flocculans NLF-7-7T (=KCTC 62943T). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Comamonas flocculans NLF-7-7T is MN527436. The whole-genome shotgun BioProject Number is PRJNA555370 with the Accession Number CP042344.
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Abbreviations
- AL:
-
Aminolipid
- DPG:
-
Diphosphatidylglycerol
- HPLC:
-
High-performance liquid chromatography
- KCTC:
-
Korean Collection for Type Cultures
- PE:
-
Phosphatidylethanolamine
- PG:
-
Phosphatidylglycerol
- TEM:
-
Transmission electron microscopy
- TLC:
-
Thin-layer chromatography
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Acknowledgements
We thank Professor Bernhard Schink for his advice in naming the novel strain.
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This research was supported by the Project for Cooperative R&D between Industry, Academy, and Research Institute funded by the Korea Ministry of SMEs and Startups in 2018 (Grant Number S2597396); and the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program.
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Kim, DH., Han, KI., Kwon, HJ. et al. Comamonas flocculans sp. nov., a Floc-Forming Bacterium Isolated from Livestock Wastewater. Curr Microbiol 77, 1902–1908 (2020). https://doi.org/10.1007/s00284-020-01940-5
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DOI: https://doi.org/10.1007/s00284-020-01940-5