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Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants

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Abstract

The relative abundance and diversity of lactobacilli present in feces of infants fed with breastmilk and fructooligosaccharide-galactooligosaccharide (FOS-GOS)-, and inulin-galactooligosaccharide (inulin-GOS)-supplemented infant formulae and combination of both were compared. Fecal lactobacilli rapidly colonized and reached maximum total cell counts, which were significantly higher in the infants fed by combining breastmilk with a formula containing either FOS-GOS (C1-A infant) or inulin-GOS (C2-C infant) and the exclusively formula fed ones (F1-F and F2-H infants) than those detected in the exclusively breast-fed (B1-D and B2-E infants) (P < 0.05). The greatest relative abundance of fecal lactobacilli species was observed in all infant receiving prebiotic-containing diets, whereas bifidobacteria appeared predominantly in exclusively breast-fed infants. The species composition of lactobacilli was highly unique among individual and more variable in both groups of infants receiving breastmilk than the exclusively formula-fed infants. Breastmilk seem to be a great source of indigenous lactobacilli vertically transferred and continuously seeded infants’ gut. Meanwhile, prebiotic supplementation in infant formulae enhanced and sustained the successful colonization of lactobacilli.

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Acknowledgements

This research was financially supported by Graduate School, Prince of Songkla University, and the Office of the Higher Education Commission under the CHE-PhD Scholarship program (Grant No. 03–2553) granted to Khanitta Kongnum under supervision of Associate Prof. Tipparat Hongpattarakere.

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  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand

    Khanitta Kongnum, Siriporn Taweerodjanakarn & Tipparat Hongpattarakere

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  1. Khanitta Kongnum
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Correspondence to Tipparat Hongpattarakere.

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Ethical Approval

This research protocol was reviewed and approved by the Ethics Committee, Faculty of Medicine, Prince of Songkla University (EC Number: 55–244-19–2-3) and (EC Number: 55–243-19–2-3). Verbal informed consent was obtained from all infants’ parents on behalf of the participating baby. The verbal consent was granted by the Ethics Committee because this research presented negligible risks to the infant participants. Moreover, all samples and data were processed and analyzed anonymously.

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Kongnum, K., Taweerodjanakarn, S. & Hongpattarakere, T. Impacts of Prebiotic-Supplemented Diets and Breastmilk on Population and Diversity of Lactobacilli Established in Thai Healthy Infants. Curr Microbiol 77, 1191–1202 (2020). https://doi.org/10.1007/s00284-020-01920-9

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  • DOI: https://doi.org/10.1007/s00284-020-01920-9

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