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cAMP Receptor Protein of Salmonella enterica Serovar Typhimurium Modulate Glycolysis in Macrophages to Induce Cell Apoptosis

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Abstract

We studied the role of glycolysis in the mechanism of cAMP receptor protein-induced macrophage cell death of Salmonella enterica serovar Typhimurium (S. Typhimurium). Cell apoptosis, caspase-3, -8, -9 enzyme activity, and pyruvic acid, lactic acid, ATP, and hexokinase (HK) contents were determined after infection of macrophages with S. Typhimurium SL1344 wild-type and a cAMP receptor protein mutant strain. While cell apoptosis, caspase-3, -8, -9 enzyme activity, lactic acid, hexokinase, and ATP levels significantly changed by infection with crp mutants compared to the wild-type strain (P < 0.05). Our data suggest that the cAMP receptor protein of S. Typhimurium can modulate macrophage death by effecting glycolysis levels. This finding may help to elucidate the mechanisms of S. Typhimurium pathogenesis.

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (Grant 31572489), the HeNan Natural Science Foundation (182300410078), and the National Key R&D Program (2016YFD0500708).

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Authors and Affiliations

  1. The Key Lab of Animal Disease and Public Healthy, Henan University of Science and Technology, Luoyang, China

    Ke Ding, Chunjie Zhang, Jing Li, Songbiao Chen, Chengshui Liao, Xiangchao Cheng, Chuang Yu, Zuhua Yu & Yanyan Jia

  2. Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, Luoyang, China

    Ke Ding, Chunjie Zhang, Jing Li, Songbiao Chen, Chengshui Liao, Xiangchao Cheng, Chuang Yu, Zuhua Yu & Yanyan Jia

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  1. Ke Ding
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Correspondence to Chunjie Zhang.

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Ding, K., Zhang, C., Li, J. et al. cAMP Receptor Protein of Salmonella enterica Serovar Typhimurium Modulate Glycolysis in Macrophages to Induce Cell Apoptosis. Curr Microbiol 76, 1–6 (2019). https://doi.org/10.1007/s00284-018-1574-1

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