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Effects of Phenolic Compounds on Growth of Colletotrichum spp. In Vitro

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Abstract

Colletotrichum acutatum is responsible for anthracnose fruit rot, one of the most devastating diseases in strawberry. Phenolic compounds have been described as contributors to anthracnose resistance in strawberry (Fragaria x ananassa, Duch.). Six isolates of Colletotrichum acutatum and four isolates of three other Colletotrichum species, C. gloeosporioides, C. fragariae, and C. graminicola, associated with disease symptoms were investigated in this study. The potential inhibitory effect of phenolic acids (gallic acid, caffeic acid, chlorogenic acid, ferulic acid, trans-cinnamic acid, p-coumaric acid, salicylic acid), flavonoids (catechin, quercetin, naringenin), and ellagic acid, which are naturally found in strawberry, were screened against two different spore suspension concentrations of the Colletotrichum isolates at 5, 10, 50 mM in vitro. Among the phenolic acids and flavonoids tested in this study, only trans-cinnamic acid, ferulic acid, and p-coumaric acid inhibited fungal growth. The inhibitory effects were concentration-dependent but also varied with the spore suspension concentration of the isolates. The results demonstrated that trans-cinnamic acid had the greatest inhibitory effect on all Colletotrichum spp. isolates tested.

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Acknowledgements

The authors would like to sincerely thank Dr. Lisa Vaillancourt, Department of Plant Pathology University of Kentucky, for providing the Colletotrichum isolates and guidance throughout this study. This is publication No. 17-11-062 of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This project was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture.

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Authors and Affiliations

  1. Department of Horticulture, University of Kentucky, Lexington, KY, 40546, USA

    Sutapa Roy & Douglas D. Archbold

  2. Department of Plant Pathology, University of Kentucky, Lexington, KY, 40546, USA

    Etta Nuckles

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  1. Sutapa Roy
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Correspondence to Douglas D. Archbold.

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Roy, S., Nuckles, E. & Archbold, D.D. Effects of Phenolic Compounds on Growth of Colletotrichum spp. In Vitro. Curr Microbiol 75, 550–556 (2018). https://doi.org/10.1007/s00284-017-1415-7

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