Abstract
Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a useful tool for rapid identification of microorganisms. Unfortunately, its direct application to positive blood culture is still lacking standardized procedures. In this study, we evaluated an easy- and rapid-to-perform protocol for MALDI-TOF MS direct identification of microorganisms from positive blood culture after a short-term incubation on solid medium. This protocol was used to evaluate direct identification of microorganisms from 162 positive monomicrobial blood cultures; at different incubation times (3, 5, 24 h), MALDI-TOF MS assay was performed from the growing microorganism patina. Overall, MALDI-TOF MS concordance with conventional methods at species level was 60.5, 80.2, and 93.8% at 3, 5, and 24 h, respectively. Considering only bacteria, the identification performances at species level were 64.1, 85.0, and 94.1% at 3, 5, and 24 h, respectively. This protocol applied to a commercially available MS typing system may represent, a fast and powerful diagnostic tool for pathogen direct identification and for a promptly and pathogen-driven antimicrobial therapy in selected cases.
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References
Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, Yeo YH, Tseng CP, Lee CC (2013) Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis—a systemic review and meta-analysis. PLoS ONE 8(5):e62323. doi:10.1371/journal.pone.0062323
Gray TJ, Thomas L, Olma T, Iredell JR, Chen SC (2013) Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry. Diagn Microbiol Infect Dis 77(2):110–112. doi:10.1016/j.diagmicrobio.2013.06.016
Idelevich EA, Becker K (2016) Identification and susceptibility testing from shortly incubated cultures accelerate blood culture diagnostics at no cost. Clin Infect Dis 62(2):268–269. doi:10.1093/cid/civ824
Idelevich EA, Schüle I, Grünastel B, Wüllenweber J, Peters G, Becker K (2014) Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium. Clin Microbiol Infect 20(10):1001–1006. doi:10.1111/1469-0691.12640
Kerremans JJ, Verboom P, Stijnen T, Hakkaart-van Roijen L, Goessens W, Verbrugh HA, Vos MC (2008) Rapid identification and antimicrobial susceptibility testing reduce antibiotic use and accelerate pathogen-directed antibiotic use. J Antimicrob Chemother 61(2):428–435 Epub 2007 Dec 21
Klouche M, Schröder U (2008) Rapid methods for diagnosis of bloodstream infections. Clin Chem Lab Med 46(7):888–908. doi:10.1515/CCLM.2008.157
Kohlmann R, Hoffmann A, Geis G, Gatermann S (2015) MALDI-TOF mass spectrometry following short incubation on a solid medium is a valuable tool for rapid pathogen identification from positive blood cultures. Int J Med Microbiol 305(4–5):469–479. doi:10.1016/j.ijmm.2015.04.004
La Scola B, Raoult D (2009) Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PLoS ONE 4(11):e8041. doi:10.1371/journal.pone.0008041
Machen A, Drake T, Wang YF (2014) Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system. PLoS ONE 9(2):e87870. doi:10.1371/journal.pone.0087870
Martinez RM, Bauerle ER, Fang FC, Butler-Wu SM (2014) Evaluation of three rapid diagnostic methods for direct identification of microorganisms in positive blood cultures. J Clin Microbiol 52(7):2521–2529. doi:10.1128/JCM.00529-14
Mestas J, Felsenstein S, Bard JD (2014) Direct identification of bacteria from positive BacT/ALERT blood culture bottles using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Diagn Microbiol Infect Dis 80(3):193–196. doi:10.1016/j.diagmicrobio.2014.07.008
Monteiro J, Inoue FM, Lobo AP, Sugawara EK, Boaretti FM, Tufik S (2015) Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the VITEK® MS system. J Microbiol Methods 109:157–159. doi:10.1016/j.mimet.2014.12.009
Paolucci M, Landini MP, Sambri V (2010) Conventional and molecular techniques for the early diagnosis of bacteraemia. Int J Antimicrob Agents 36(Suppl 2):S6–16. doi:10.1016/j.ijantimicag.2010.11.010
Paul M, Shani V, Muchtar E, Kariv G, Robenshtok E, Leibovici L (2010) Systematic review and meta-analysis of the efficacy of appropriate empiric antibiotic therapy for sepsis. Antimicrob Agents Chemother 54(11):4851–4863. doi:10.1128/AAC.00627-10
Verroken A, Defourny L, Lechgar L, Magnette A, Delmée M, Glupczynski Y (2015) Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture. Eur J Clin Microbiol Infect Dis 34(2):405–413. doi:10.1007/s10096-014-2242-4
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“All procedures performed in studies involving human participants where in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.”
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Antonio Curtoni and Raffaella Cipriani equally contributed to this study and share first authorship.
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Curtoni, A., Cipriani, R., Marra, E.S. et al. Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium. Curr Microbiol 74, 97–102 (2017). https://doi.org/10.1007/s00284-016-1161-2
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DOI: https://doi.org/10.1007/s00284-016-1161-2