Abstract
Photorhabdus luminescens subsp. laumondii is closely associated with the entomopathogenic nematode Heterorhabditis bacteriophora and has, to date, not been isolated from other nematode species. This study is the first report of P. luminescens subsp. laumondii from two South African isolates of entomopathogenic nematodes, Heterorhabditis safricana SF281 and H. bacteriophora SF351. Both symbiotic bacterial strains are phenotypically closely related to P. luminescens subsp. laumondii previously isolated and described from H. bacteriophora. The genetic relatedness between P. luminescens subsp. laumondii strains SF281B and SF351B was confirmed by comparing 16S rDNA, recA, gyrB and gltX sequences with sequences of P. luminescens subsp. laumondii, including the type strain (TT01T) and strain E21.
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Acknowledgments
The Central Analytical Facility (CAF) of Stellenbosch University for DNA sequencing. The research was supported with funding from the National Research Foundation (NRF), South Africa (Grant number NRF-THRIP TP14062571871) and the South African Apple and Pear Producers’ Association (SAAPPA).
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284_2016_1116_MOESM1_ESM.tif
Bioluminescence produced by Photorhabdus luminescens subsp. laumondii SF281B after 48 h of growth at 30°C on MacConkey agar (Biolab) (TIFF 336 kb)
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Geldenhuys, J., Malan, A.P. & Dicks, L.M.T. First Report of the Isolation of the Symbiotic Bacterium Photorhabdus luminescens subsp. laumondii Associated with Heterorhabditis safricana from South Africa. Curr Microbiol 73, 790–795 (2016). https://doi.org/10.1007/s00284-016-1116-7
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DOI: https://doi.org/10.1007/s00284-016-1116-7