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The Detection of Inherent Homologous Recombination Between Repeat Sequences in H. pylori 26695 by the PCR-Based Method

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Abstract

Helicobacter pylori infects more than half of the world’s population, making it the most widespread infection of bacteria. It has high genetic diversity and has been considered as one of the most variable bacterial species. In the present study, a PCR-based method was used to detect the presence and the relative frequency of homologous recombination between repeat sequences (>500 bp) in H. pylori 26695. All the recombinant structures have been confirmed by sequencing. The inversion generated between inverted repeats showed distinct features from the recombination for duplication or deletion between direct repeats. Meanwhile, we gave the mathematic reasoning of a general formula for the calculation of relative recombination frequency and indicated the conditions for its application. This formula could be extensively applied to detect the frequency of homologous recombination, site-specific recombination, and other types of predictable recombination. Our results should be helpful for better understanding the genome evolution and adaptation of bacteria.

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Acknowledgments

The authors are very grateful to Dr. Nina R. Salama for her kind help in providing them some genomic DNA of Helicobacter pylori. Xianwu Guo and Mario A. Rodríguez-Pérez hold a scholarship from Comisión de Operación y Fomento de Actividades Académicas/Instituto Politécnico Nacional. This work was supported by the Consejo Nacional de Ciencia y Tecnología-México (http://www.conacyt.gob.mx/convocatorias/Paginas/default.aspx) (Fondo Sectorial de Investigación Básica SEP CONACyT (CB-2011-01) with Grant number: 168541); and Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional, México (http://www.sip.ipn.mx/WPS/WCM/CONNECT/SIP/SIP/INICIO/INDEX.HTM) (Grant numbers: SIP20120202).

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Authors and Affiliations

  1. Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Boulevard del Maestro S/N esq. Elías Piña, Col. Narciso Mendoza, Cd., 88710, Reynosa, Tamaulipas, Mexico

    Yajuan Fu, Reyna Cristina Zepeda-Gurrola, Edgar E. Lara-Ramírez, Erick J. De Luna-Santillana, Isabel Cristina Rodríguez-Luna, Alejandro Sánchez-Varela, Víctor Ricardo Moreno-Medina, Mario A. Rodríguez-Pérez & Xianwu Guo

  2. Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Instituto Politécnico Nacional, Altamira, Tamaulipas, Mexico

    Yajuan Fu

  3. Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico

    Germán Rubén Aguilar-Gutiérrez

  4. Centro de Investigaciones en Ciencias Microbiológicas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico

    Ricardo Carreño-López

  5. Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F., Mexico

    Yolanda López-Vidal

Authors
  1. Yajuan Fu
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  2. Reyna Cristina Zepeda-Gurrola
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  3. Germán Rubén Aguilar-Gutiérrez
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  4. Edgar E. Lara-Ramírez
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  5. Erick J. De Luna-Santillana
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  6. Isabel Cristina Rodríguez-Luna
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  7. Alejandro Sánchez-Varela
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  8. Ricardo Carreño-López
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  9. Víctor Ricardo Moreno-Medina
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  10. Mario A. Rodríguez-Pérez
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  11. Yolanda López-Vidal
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  12. Xianwu Guo
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Corresponding author

Correspondence to Xianwu Guo.

Additional information

Yajuan Fu and Reyna Cristina Zepeda-Gurrola have contributed equally to this study.

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Fu, Y., Zepeda-Gurrola, R.C., Aguilar-Gutiérrez, G.R. et al. The Detection of Inherent Homologous Recombination Between Repeat Sequences in H. pylori 26695 by the PCR-Based Method. Curr Microbiol 68, 211–219 (2014). https://doi.org/10.1007/s00284-013-0466-7

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  • DOI: https://doi.org/10.1007/s00284-013-0466-7

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