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Isolation and Characterization of a Lytic Myoviridae Bacteriophage PAS-1 with Broad Infectivity in Aeromonas salmonicida

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Abstract

To search for candidate control agents against Aeromonas salmonicida subsp. salmonicida infections in aquaculture, one bacteriophage (phage), designated as PAS-1, was isolated from the sediment samples of the rainbow trout (Oncorhynchus mykiss) culture farm in Korea. The PAS-1 was morphologically classified as Myoviridae and possessed approximately 48 kb of double-strand genomic DNA. The phage showed broad host ranges to other subspecies of A. salmonicida as well as A. salmonicida subsp. salmonicida including antibiotic-resistant strains. Its latent period and burst size were estimated to be approximately 40 min and 116.7 PFU/cell, respectively. Furthermore, genomic and structural proteomic analysis of PAS-1 revealed that the phage was closely related to other Myoviridae phages infecting enterobacteria or Aeromonas species. The bacteriolytic activity of phage PAS-1 was evaluated using three subspecies of A. salmonicida strain at different doses of multiplicity of infection, and the results proved to be efficient for the reduction of bacterial growth. Based on these results, PAS-1 could be considered as a novel Aeromonas phage and might have potentiality to reduce the impacts of A. salmonicida infections in aquaculture.

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Acknowledgments

This study was financially supported by Basic Science Research Program (2010-0016748) and Priority Research Centers Program (2009-0093822) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and by Korea Research Foundation Grant (KRF-2008-331-E00385).

Conflict of Interest

The author(s) declare that they have no competing interests.

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Authors and Affiliations

  1. Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea

    J. H. Kim, C. H. Choresca, S. P. Shin, J. E. Han, J. W. Jun & S. C. Park

  2. Basic Science Institute for Cell Damage Control, Sogang University, Seoul, 121-742, Republic of Korea

    J. S. Son

  3. Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-742, Republic of Korea

    Y. J. Choi

  4. Korea Ocean Research & Development Institute, Ansan, 426-744, Republic of Korea

    J. H. Kim, D. H. Kang, C. Oh & S. J. Heo

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  1. J. H. Kim
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  2. J. S. Son
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  3. Y. J. Choi
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  4. C. H. Choresca
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  5. S. P. Shin
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  6. J. E. Han
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  7. J. W. Jun
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  8. D. H. Kang
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  9. C. Oh
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  10. S. J. Heo
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  11. S. C. Park
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Corresponding author

Correspondence to S. C. Park.

Additional information

J. H. Kim and J. S. Son have contributed equally to this work.

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Kim, J.H., Son, J.S., Choi, Y.J. et al. Isolation and Characterization of a Lytic Myoviridae Bacteriophage PAS-1 with Broad Infectivity in Aeromonas salmonicida . Curr Microbiol 64, 418–426 (2012). https://doi.org/10.1007/s00284-012-0091-x

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  • DOI: https://doi.org/10.1007/s00284-012-0091-x

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