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Enhancement of Fermentative Hydrogen Production in an Extreme-Thermophilic (70°C) Mixed-Culture Environment by Repeated Batch Cultivation

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Abstract

Repeated batch cultivation was applied to enrich hydrogen fermentative microflora under extreme-thermophilic (70°C) environment. Initial inoculums received from a hydrogen producing reactor fed with organic fraction of household solid wastes. In total seven transfers was conducted and maximum hydrogen yield reached 296 ml H2/g (2.38 mol/mol) glucose and 252 ml H2/g (2.03 mol/mol) for 1 and 2 g/l glucose medium, respectively. It was found that hydrogen production was firstly decreased and got increased gradually from third generation. Acetate was found to be the main metabolic by-product in all batch cultivation. Furthermore, the diversity of bacterial community got decreased after repeated batch cultivation. It was proved that repeated batch cultivation was a good method to enhance the hydrogen production by enriching the mixed cultures of dominant species.

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Acknowledgements

The research was founded by Sino-Danish Scientific & Technological Cooperation Program (no. AM15:26) and National Natural Science Foundation of China (Grant no. 41101225).

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Authors and Affiliations

  1. School of Enviornment, Tsinghua University, Beijing, 100084, China

    Wenjing Lu, Gaoyuan Fan, Chenxi Zhao, Hongtao Wang & Zifang Chi

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  1. Wenjing Lu
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  2. Gaoyuan Fan
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  3. Chenxi Zhao
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  4. Hongtao Wang
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Correspondence to Wenjing Lu.

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Lu, W., Fan, G., Zhao, C. et al. Enhancement of Fermentative Hydrogen Production in an Extreme-Thermophilic (70°C) Mixed-Culture Environment by Repeated Batch Cultivation. Curr Microbiol 64, 427–432 (2012). https://doi.org/10.1007/s00284-012-0088-5

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  • DOI: https://doi.org/10.1007/s00284-012-0088-5

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