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Cohnella ferri sp. nov. A Novel Member of the Genus Cohnella Isolated from Haematite Ore

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Abstract

The taxonomic position of a Gram-positive, endo-spore forming bacterium isolated from a haematite ore sample was analyzed by a polyphasic approach. The strain designated as HIO-4T matched most of the phenotypic and chemical characteristics of the genus Cohnella and represents a novel species. The sequence of the almost complete 16S rRNA (1489 bases) was compared with those of previously studied Cohnella type strains and confirmed that the strain belongs to the genus Cohnella. Strain HIO-4T differs from all other species of Cohnella by at least 3.9% at the 16S rRNA level and the moderately related species are Cohnella phaseoli (96.1%) and Cohnella yongneupensis (96.1%), respectively. Predominant polar lipids are diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE); few unknown phospholipids, mannose containing lipid, aminophospholipid and aminophosphoglycolipids. The results of physiological and biochemical tests allowed the genotypic and phenotypic distinctiveness of strain HIO-4T with its phylogenetic relatives and suggest that the strain HIO-4T should be recognized as a novel species, for which the name Cohnella ferri sp. nov. is proposed. The type strain is HIO-4T (=MTCC 8365T = JCM 16139T)

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Acknowledgments

We thank Professor J P Euzéby, Ecole Nationale Veterinaire, France for his suggestion on Latin nomenclature for the novel species. I would like to thank Ms Anju Choudhary, Ms Deepika Sharma and Mr Malkit Singh for their excellent technical assistance and Ms Varpreet Kaur for getting the iron ore samples. AR is a recipient of DBT Postdoctoral fellowship. Financial assistance from CSIR and DBT, Government of India is duly acknowledged. This is IMTECH communication number 084/2010.

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Author notes

  1. Arunachalam Ruckmani

    Present address: Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India

Authors and Affiliations

  1. MTCC-Microbial Type Culture Collection & Gene Bank, Institute of Microbial Technology, Chandigarh, 160036, India

    Shanmugam Mayilraj, Arunachalam Ruckmani, Chandandeep Kaur & Ishwinder Kaur

  2. DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany

    Hans-Peter Klenk

Authors
  1. Shanmugam Mayilraj
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  2. Arunachalam Ruckmani
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  3. Chandandeep Kaur
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  4. Ishwinder Kaur
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  5. Hans-Peter Klenk
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Corresponding author

Correspondence to Shanmugam Mayilraj.

Additional information

The GenBank accession number for the 16S rRNA sequence of strain HIO-4T is EF 203083.

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Mayilraj, S., Ruckmani, A., Kaur, C. et al. Cohnella ferri sp. nov. A Novel Member of the Genus Cohnella Isolated from Haematite Ore. Curr Microbiol 62, 1704–1709 (2011). https://doi.org/10.1007/s00284-011-9917-1

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  • DOI: https://doi.org/10.1007/s00284-011-9917-1

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