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Oral Administration of Live Bifidobacterium Substrains Isolated from Centenarians Enhances Intestinal Function in Mice

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Abstract

We studied the effects of two bifidobacteria strains isolated from healthy centenarians on intestinal function in mice. Bifidobacterium adolescentis BBMN23 and Bifidobacterium longum BBMN68 were orally administrated to specific pathogen-free BALB/c mice at different doses (2 × 1011, 2 × 109, or 2 × 107 CFU/kg body weight) each day for 4 weeks. Villus height, crypt depth, villus width, and villus/crypt ratio (V/C) were determined. The content of duodenal secreted immunoglobulin A (sIgA) was also evaluated. There were clear increases in height and width of duodenal villi in both treated groups. Crypt depths were deeper in animals treated with BBMN23 than in controls, while depths were reduced in animals receiving BBMN68. The V/C ratio was increased after feeding with BBMN68, while BBMN23 had no significant effect. Both strains improved the sIgA content of the duodenum. These results suggest that BBMN23 and BBMN68 may improve intestinal digestion and ability and enhance immune barrier function in the intestine.

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Acknowledgments

This study was financially supported by the Mega-Projects of Science Research for the 11th Five-Year Plan (Item No. 2006BAD04A06) and the 863 Plan (Item No. 2007AA10Z311) of the Ministry of Science and Technology of China. We thank Li Cao (Institute of Medicinal Plant Development, Beijing, China) for providing reagents and technical assistance; Yan Liu, Wei Wei, and Yan Guo (Institute of Medicinal Plant Development, Beijing, China) for care and sampling of animals; Ruiping She and Ye Ding (College of Veterinary Medicine, China Agricultural University, Beijing, China) for preparation of specimens; Lu Jiang, Xueying Mao (China Agricultural University, Beijing, China), and Kenneth J. Gruber (School of Agricultural and Environmental Sciences, North Carolina A&T State University) for critical reading of the manuscript.

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China

    Haiying Yang, Ming Zhang, Zhihua Pang & Xiaojing Leng

  2. MengNiu Diary Company, Beijing, China

    Aiping Liu

  3. Food Microbiology and Biotechnology Laboratory, North Carolina A & T State University, Greensboro, NC, USA

    Salam A. Ibrahim

  4. Key Laboratory of Functional Dairy, College of Food Science & Nutritional Engineering, Beijing, China

    Fazheng Ren

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  1. Haiying Yang
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  2. Aiping Liu
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  3. Ming Zhang
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  4. Salam A. Ibrahim
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  5. Zhihua Pang
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  6. Xiaojing Leng
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  7. Fazheng Ren
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Correspondence to Fazheng Ren.

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Yang, H., Liu, A., Zhang, M. et al. Oral Administration of Live Bifidobacterium Substrains Isolated from Centenarians Enhances Intestinal Function in Mice. Curr Microbiol 59, 439–445 (2009). https://doi.org/10.1007/s00284-009-9457-0

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  • DOI: https://doi.org/10.1007/s00284-009-9457-0

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