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Study of the Adhesion of Bifidobacterium bifidum MIMBb75 to Human Intestinal Cell Lines

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Abstract

The aim of this study was to investigate the adhesive phenotype of the human intestinal isolate Bifidobacterium bifidum MIMBb75 to human colon carcinoma cell lines. We have previously shown that the adhesion of this strain to Caco-2 cells is mediated by an abundant surface lipoprotein named BopA. In this study, we found that this strain adheres to Caco-2 and HT-29 cells, and that its adhesion strongly depends on the environmental conditions, including the presence of sugars and bile salts and the pH. Considerably more adhesion to a Caco-2 monolayer occurred in the presence of fucose and mannose and less when MIMBb75 grew in Oxgall bile salts compared to standard environmental conditions. In particular, growth in Oxgall bile salts reduced the adhesion ability of MIMBb75 and modified the SDS-PAGE profile of the cell wall associated proteins of the strain. The pH markedly affected both adhesion to Caco-2 and bacterial autoaggregation. Finally, experiments with sodium metaperiodate suggested that not only proteinaceous determinants are involved in the adhesion process of B. bifidum. In conclusion, it seems that the colonization strategy of this bacterium can be influenced by factors varying along the gastrointestinal tract, such as the presence of specific sugars and bile salts and the pH, possibly limiting the adhesion of B. bifidum to only restricted distal sites of the gut.

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Acknowledgment

This work has been partially funded by the Italian Government grant PUR 2008.

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Authors and Affiliations

  1. Department of Food Science and Microbiology, University of Milan, Milan, Italy

    Simone Guglielmetti, Isabella Tamagnini, Stefania Arioli, Carlo Parini & Diego Mora

  2. Department of Biomolecular Sciences and Biotechnology, University of Milan, Milan, Italy

    Mario Minuzzo

  3. Department of Nutritional Sciences, University of Toronto, Toronto, Canada

    Elena Comelli

Authors
  1. Simone Guglielmetti
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  2. Isabella Tamagnini
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  3. Mario Minuzzo
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  4. Stefania Arioli
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  5. Carlo Parini
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  6. Elena Comelli
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  7. Diego Mora
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Correspondence to Simone Guglielmetti.

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Guglielmetti, S., Tamagnini, I., Minuzzo, M. et al. Study of the Adhesion of Bifidobacterium bifidum MIMBb75 to Human Intestinal Cell Lines. Curr Microbiol 59, 167–172 (2009). https://doi.org/10.1007/s00284-009-9415-x

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  • DOI: https://doi.org/10.1007/s00284-009-9415-x

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