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First Confirmation of Integron-Bearing Methicillin-Resistant Staphylococcus aureus

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Abstract

Six methicillin-resistant Staphylococcus aureus MRSA strains from two nosocomial infection cases described in a previous study [15], of which two ocurred in March and the other four in May, 2005, were found to possess one copy of class 1 integron with aadA2 gene cassette located on chromosomes by Southern hybridization. Polymerase chain reaction (PCR) detection of mecA and pvl, SCCmec typing, multilocus sequence typing (MLST), spaA typing and coa typing were also performed. The results revealed 6 MRSA fell into the ST239-MRSA-III group (clonal complex 8), with the spaA type GKAOMQ and coa type HIJKL, whereas the pvl locus was not detected. DNA fingerprinting analysis by random amplified polymorphic DNA–PCR using three different assays were also performed, and all strains exhibited identical patterns, indicating that they were clonally related and might be mainly due to a specific clone in the hospital. This was the first time, to our knowledge, that class 1 integron-bearing MRSA (I-MRSA), simultaneously carrying two mobile genetic elements was confirmed: class 1 integron and SCCmec.

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Acknowledgments

We thank T. Ito and K. Hiramatsu for providing us the SCCmec type strains of MRSA.

This work was supported by National Natural Science Foundation of China (20436020).

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Authors and Affiliations

  1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China

    Zhenbo Xu, Lin Li, Shinji Yamasaki & Lei Shi

  2. Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS, 66506, USA

    M. J. Alam

  3. Department of Medical Pathology, Guangdong Provincial People’s Hospital, Guangzhou, China

    Liyan Zhang

  4. Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan

    Shinji Yamasaki & Lei Shi

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  1. Zhenbo Xu
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  2. Lin Li
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  3. M. J. Alam
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  4. Liyan Zhang
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  5. Shinji Yamasaki
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  6. Lei Shi
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Correspondence to Lei Shi.

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Xu, Z., Li, L., Alam, M.J. et al. First Confirmation of Integron-Bearing Methicillin-Resistant Staphylococcus aureus . Curr Microbiol 57, 264–268 (2008). https://doi.org/10.1007/s00284-008-9187-8

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  • DOI: https://doi.org/10.1007/s00284-008-9187-8

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