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Easy Visualization of the Protist Oxyrrhis marina Grazing on a Live Fluorescently Labelled Heterotrophic Nanoflagellate

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Abstract

Planktonic heterotrophic flagellates are ubiquitous eukaryotic microorganisms that play a crucial role in carbon and nutrient fluxes through pelagic food webs. Here we illustrate for the first time a grazing model of planktonic dinoflagellate, Oxyrrhis marina, on the heterotrophic nanoflagellate Goniomonas amphinema, using the DNA-binding fluorescent dye Hoechst 33342. A solution of 1 μg/mL of the fluorochrome allowed viability of the prey for at least 48 hours, provided low fluorescence quenching, and labelled the flagellate without masking the cytoplasm. After 2 hours of contact between the fluorescent prey and the predator, O. marina population had preyed on live G. amphinema at an ingestion rate of 2.2 prey Oxyrrhis −1 h−1. Results show that this model is a time-effective and inexpensive approach for the direct observation of heterotrophic flagellate grazing. The fact that prey remain alive while predation occurs, as well as the low rate of quenching, could be of help in studying the fate of real-time trophic interactions between protists in microbial webs.

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Acknowledgment

This study was supported by a Marie Curie Fellowship of the European Community under Contract No. HPMF-CT-2002-01861 to M. M-C.

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Authors and Affiliations

  1. Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, KS, 66045, USA

    Mercedes Martín-Cereceda

  2. Biodiversity Research Center, University of Kansas, Lawrence, KS, 66045, USA

    Richard A. J. Williams

  3. Department of Zoology, Natural History Museum, London, SW7 5BD, UK

    Gianfranco Novarino

Authors
  1. Mercedes Martín-Cereceda
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  2. Richard A. J. Williams
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  3. Gianfranco Novarino
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Correspondence to Mercedes Martín-Cereceda.

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Martín-Cereceda, M., Williams, R.A.J. & Novarino, G. Easy Visualization of the Protist Oxyrrhis marina Grazing on a Live Fluorescently Labelled Heterotrophic Nanoflagellate. Curr Microbiol 57, 45–50 (2008). https://doi.org/10.1007/s00284-008-9150-8

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  • DOI: https://doi.org/10.1007/s00284-008-9150-8

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