Abstract
Soil microcosm experiments were used to investigate the effects on growth and activity of soil microorganisms of an herbicide combination (60% bromoxynil + 3% prosulfuron) frequently used to provide a broad spectrum control of weed species. Culturable aerobic bacteria, fungi, and actinomycetes, the fundamental groups of heterotrophic microorganisms, and nitrifiers, considered a very sensitive group to these compounds, were evaluated. Since herbicides have been found to inhibit decomposition of cellulose in soil, the effects on cellulolytic bacteria and fungi were determined. Dehydrogenase activity as a measure of microbial activity was another parameter considered. The results emphasized a tendency of reversible stimulatory/inhibitory effects of the tested compounds on soil microorganisms, with fungi as an exception. A long-lasting negative action on the activity of the dehydrogenase (DHA), commonly used as an index of the overall microbial activity in soil, was found. The magnitude of these effects were dependent on the assayed concentrations of the herbicides mixture. We concluded that the presence of bromoxynil + prosulfuron could induce significant changes in the microbial populations of the soil, concerning the activity and balance of microbial community. Possible environmental risks must be considered. Dehydrogenase activity was shown to be an important indicator of side-effects attributed to these herbicides.
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Literature Cited
Ahtiainen JH, Vanhala P, Myllymaki A (2003) Effects of different plant protection programs on soil microbes. Ecotoxicol Environ Safety 54:56–64
Allievi L, Gigliotti C (2001) Response of the bacteria and fungi of two soils to the sulfonylurea herbicide cinosulfuron. J Environ Sci Health B36:(2)161–175
Barnet M, Hodgson B (1991) Differential effects of the sulfonylurea herbicides chlorsulfuron and sulfometuron methyl on microorganisms. Arch Microbiol 155:521–525
Beyer EM, Duffy MJ, Hay JV, Schlueter DD (1988) Sulfonylurea herbicides. In: Kearney PC, Kaufman DD (eds) Herbicides: Chemistry degradation and mode of action. New York: Marcel Dekker, pp 125–140
Blair AM, Martin TD (1988) A review of the activity and mode of action of sulfonylurea herbicides. Pesticide Sci 22:195–219
Boldt T, Jacobsen CS (1998) Differential toxic effects of the sulfonylurea herbicides metsulfuron methyl, chlorsulfuron and thifensulfuron methyl on fluorescent pseudomonads isolated from on agricultural soil. FEMS Microbiol Lett 161:29–35
Brown HM (1990) Mode of action, crop selectivity and soil relations of the sulfonylurea herbicides. Pestic Sci 29:263–281
Camiña F, Trasar-Cepeda C, Gil-Sofres F, Leirós C (1998) Measurement of dehydrogenase activity in acid soils rich in organic matter. Soil Biol Biochem 30:1005–1011
Chaleff RS, Mauvis CJ (1984) Acetolactate synthase as the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443–1445
Chen SK, Edwards CA, Subler S (2001) Effects of the fungicides benomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biol Biochem 33:1971–1980
Dumontet S, Perucci P, Scopa A, Ricciardi A (1993) Sulfonylureas: preliminary study on the effect on selected microbial strains and soil respiration. Soil Sci (Trends Agric Sci) 1:193–198
Edwards IR, Ferry DG, Temple WA (1991) Fungicides and related compounds. In: Hayes WJ, Laws ER (eds) Handbook of pesticide toxicology. New York: Academic Press pp. 1436–1451
Forlani G, Mantelli M, Branzoni M, Nielsen E, Favilli F (1995) Differential sensitivity plant associated bacteria to sulfonylurea and imidazolinone herbicides. Plant Soil 243–253
Gigliotti C, Allier L, Salardi C, Ferrari F, Farini A (1998) Microbial ecotoxicity and persistence in soil of the herbicide bensulfuron-methyl. J Environ Sci Health B33(4):381–398
Ismail BS, Yap MY (1994) Effects of two acetanilide herbicides on microbial populations and their cellulolytic activities. Bull Environ Contam Toxicol 52:61–68
Johnen BG, Drew EA (1977) Ecological effects of pesticides on soil microorganisms. Soil Sci 123:319–324
Kidd H, James DR (1991) The agrochemicals handbook, Cambridge, UK: Royal Society of Chemistry Information Services Cambridge, UK
Kulowski K, Zirbes EL, Thede BM, Rosazza JPN (1997) Microbial transformation of prosulfuron. J Agric Food Chem 45:1479–1485
La Rossa RA, Smulski DR (1984) ilvB-encoded acetolactate synthase is resistant to the herbicide sulfometuron methyl. J Bacteriol 160: 391–394
Perucci P, Vischetti C, Battistoni F (1999) Rimsulfuron in a silty clay loam soil: effects upon microbiological and biochemical properties under varying microcosm conditions. Soil Biol Biochem 31:195–204
Peterson MA, Arnold WE (1985) Response of rotational crops to soil residue of chlorsulfuron. Weed Sci 34:131–136
Pochon J, Tardieux P (1962) Techniques d’analyse en microbiologie du sol. St Mandê, France: Editions de la Tourelle, pp. 58–62
Ratnayake M, Audus LJ (1978) Studies on the effects of herbicides on soil nitrification. Pest Biochem Physiol 8:170–185
Schloter M, Dilly O, Munch JC (2003) Indicators for evaluating soil quality. Agric Ecosyst Environ 98:255–262
Schneiders GE, Koeppe MK, Naidu MV, Horne P, Brown AM, Mucha CF (1993) Fate of rimsulfuron in the environment. J Agric Food Chem 41:2404–2410
Smith EA (1980) An analytical procedure for bromoxynil and its octanate in soils; persistence studies with bromoxynil octanoate in combination with other herbicides in soil. Pest Sci 11:341–346
Tabatabai MA (1982) Soil Enzymes. In: Page AL, Miller RH, Keeney AR (eds) Methods of soil analysis. II. Chemical and microbiological properties. Madison, American Society of Agronomy, pp. 903–947
Věková J, Pavlü L, Vosáhlo J, Gabriel J (1995) Degradation of bromoxynil by resting and immobilized cells of Agrobacterium radiobacter 8/4 strain. Biotechnol Lett 17:449–452
Vosáhlová J, Pavlü L, Vosáhlo J, Brenner V (1997) Degradation of bromoxynil, ioxynil dichlobenil and their mixtures by Agrobacterium radiobacter 8/4. Pestic Sci 49:303–306
Weber JB, Peter CJ (1982) Adsorption, bioactivity and evaluation of soil tests for alachlor acetochlor and metolachlor. Weed Sci 30:14–20
Williams ST, Wellington EMH (1982) Actinomycetes. In: Page AL, Miller RH, Keeney AR (eds) Methods of soil analysis. II. Chemical and microbial properties. Madison, WI: American Society of Agronomy Inc., pp. 969–987
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Pampulha, M.E., Oliveira, A. Impact of an Herbicide Combination of Bromoxynil and Prosulfuron on Soil Microorganisms. Curr Microbiol 53, 238–243 (2006). https://doi.org/10.1007/s00284-006-0116-4
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DOI: https://doi.org/10.1007/s00284-006-0116-4