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Effect of the Microbial Feed Additive Saccharomyces cerevisiae CNCM I-1077 on Protein and Peptide Degrading Activities of Rumen Bacteria Grown In Vitro

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Abstract

We investigated the potential of the ruminant feed additive Saccharomyces cerevisiae CNCM I-1077 on protein and peptide degrading activities of the rumen bacterial species Prevotella albensis M384, Streptococcus bovis 20480, and Butyrivibrio fibrisolvens 3071 grown in vitro. Alive or heat-killed yeast cells were added to bacterial cultures in a complex casein–glucose medium. After incubation of the cultures at 39°C under O2-free CO2, peptidase activities were determined in the absence or in the presence of yeasts. Protease activities were detected after PAGE in gelatin-copolymerized gels. In co-incubations of bacteria and live S. cerevisiae I-1077, proteinase activities were reduced compared to the activities in the bacterial monocultures. Measurement of peptidase activities and microbial enumerations in the co-incubations suggested that live yeasts and bacteria interacted in a competitive way, leading to a decrease in peptidase activities. The mechanism responsible for such an effect could be mainly a competition for substrate utilization, but the release of small competitive peptides by the yeast cells is also likely to be implicated.

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Acknowledgments

The authors thank Fabienne Floret and Brigitte Girard for their skilled technical assistance.

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Authors and Affiliations

  1. Lallemand Animal Nutrition, Sébastien Masséglia, 19 rue des Briquetiers, BP59, cedex, Blagnac, 31702, France

    Frédérique Chaucheyras-Durand & Sébastien Masséglia

  2. Unité de Microbiologie, INRA CR Clermont-Ferrand/Theix, Champanelle, Saint-Genès, 63122, France

    Frédérique Chaucheyras-Durand, Sébastien Masséglia & Gérard Fonty

  3. Laboratoire de Biologie des Protistes, UMR CNRS 6533, Université Blaise Pascal, Campus des Cézeaux, cedex, Aubière, 63177, France

    Gérard Fonty

Authors
  1. Frédérique Chaucheyras-Durand
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  2. Sébastien Masséglia
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  3. Gérard Fonty
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Correspondence to Frédérique Chaucheyras-Durand.

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Chaucheyras-Durand, F., Masséglia, S. & Fonty, G. Effect of the Microbial Feed Additive Saccharomyces cerevisiae CNCM I-1077 on Protein and Peptide Degrading Activities of Rumen Bacteria Grown In Vitro. Curr Microbiol 50, 96–101 (2005). https://doi.org/10.1007/s00284-004-4433-1

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  • DOI: https://doi.org/10.1007/s00284-004-4433-1

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