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Chitinase-Mediated Inhibitory Activity of Brassica Transgenic on Growth of Alternaria brassicae

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Abstract

Chitinase, capable of degrading the cell walls of invading phytopathogenic fungi, plays an important role in plant defense response, particularly when this enzyme is overexpressed through genetic engineering. In the present study, Brassica plant (Brassica juncea L.) was transformed with chitinase gene tagged with an overexpressing promoter 35 S CaMV. The putative transgenics were assayed for their inhibitory activity against Alternaria brassicae, the inducer of Alternaria leaf spot of Brassica both in vitro and under polyhouse conditions. In in vitro fungal growth inhibition assays, chitinase inhibited the fungal colony size by 12-56% over the non-trangenic control. The bioassay under artificial epiphytotic conditions revealed the delay in the onset of disease as well as reduced lesion number and size in 35S-chitinase Brassica as compared to the untransformed control plants.

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Authors and Affiliations

  1. Choudhary Swaran Kumar Himachal Pradesh Agricultural University, Research Station, Sangla Valley, Kuppa (Zira Farm), Kinnaur-172 106, Himachal Pradesh, India, IN

    Kalyan K. Mondal

  2. Division of Plant Pathology, Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, India, IN

    Subhas Chandra Chatterjee

  3. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, India, IN

    Navin Viswakarma, Ram Charan Bhattacharya & Anita Grover

Authors
  1. Kalyan K. Mondal
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  2. Subhas Chandra Chatterjee
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  3. Navin Viswakarma
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  4. Ram Charan Bhattacharya
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  5. Anita Grover
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Received: 23 September 2002 / Accepted: 24 October 2002

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Mondal, K., Chatterjee, S., Viswakarma, N. et al. Chitinase-Mediated Inhibitory Activity of Brassica Transgenic on Growth of Alternaria brassicae . Curr Microbiol 47, 0171–0173 (2003). https://doi.org/10.1007/s00284-002-3980-6

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  • DOI: https://doi.org/10.1007/s00284-002-3980-6

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