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Preventive effect of free radical scavenger edaravone lotion on cyclophosphamide chemotherapy-induced alopecia

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Abstract

Purpose

We investigated the inhibitory effect of edaravone (EDR) lotion on chemotherapy-induced alopecia (CIA) to improve the quality of life for patients with cancer.

Methods

Wistar rats were intraperitoneally injected with cyclophosphamide (CPA, 75 mg/kg) to induce CIA and divided into six groups: (1) Control; (2) EDR 0%; (3) EDR 0.3%; (4) EDR 3%. The TUNEL-positive area was examined histologically, and mRNA expression levels of the apoptosis-related factors, such as B-cell/CLL lymphoma 2 (Bcl-2), and Bcl-2-associated X protein (Bax), were determined.

Results

In the three CPA-treated groups, a decrease in the coverage score (percentage of hairs covered) was observed from days 16 to 18. In addition, coverage scores on day 21, the last day of observation, showed a tendency for the suppression of hair loss to increase, though hair loss was observed in all groups. The coverage scores of the EDR 0.3% and 3% groups after day 17 were significantly higher than those of the EDR 0% group. The TUNEL-positive area of skin tissue on day 16 was extensive in the EDR 0% group and decreased in the EDR 0.3% and 3% groups. The mRNA expression ratio of Bcl-2/Bax on day 21 was maintained at the same level as that of the control group only in the EDR 3% group.

Conclusion

This study confirmed the use of EDR lotion to inhibit hair loss, indicating that the clinical application of EDR lotion may improve the quality of life for patients with cancer and their willingness to undergo treatment.

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Data availability

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka, 573-0101, Japan

    Takumi Tsuji, Yu Igawa, Erika Minamino, Nodoka Otani & Yuya Yoshida

  2. Department of Pharmacy, Nara City Hospital, 1-50-1 Higashikidera-cho, Nara, Nara, 630-8305, Japan

    Katsuaki Yoneda

  3. Research Institute for Production and Development, 15 Shimogamomorimoto-cho, Sakyo-ku, Kyoto-shi, Kyoto, Japan

    Takeyuki Kohno

Authors
  1. Takumi Tsuji
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  2. Katsuaki Yoneda
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  3. Yu Igawa
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  4. Erika Minamino
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  5. Nodoka Otani
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  6. Yuya Yoshida
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  7. Takeyuki Kohno
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Contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and analyses were performed by Katsuaki Yoneda, Yu Igawa, Erika Minamino, Nodoka Otani, and Takumi Tsuji. The first draft of the manuscript was written by Takumi Tsuji, and all authors commented on previous versions of the manuscript. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Takumi Tsuji.

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This study was conducted in accordance with the regulations for animal experiments at Setsunan University (approval Nos. K20-19 and K21-20).

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Tsuji, T., Yoneda, K., Igawa, Y. et al. Preventive effect of free radical scavenger edaravone lotion on cyclophosphamide chemotherapy-induced alopecia. Cancer Chemother Pharmacol (2024). https://doi.org/10.1007/s00280-024-04669-1

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