Abstract
In a large proportion of patients with mild bleeding disorders (MBDs) no diagnosis can be established by routine coagulation tests. We investigated whether alterations in plasma clot properties account for MBDs of unknown cause. Ninety-five patients with MBDs of unknown origin and 98 age- and sex-matched healthy controls were investigated. Furthermore, data of 25 patients with a deficiency of factor VIII were analyzed. Plasma clot characteristics in the absence and presence of recombinant tissue plasminogen activator (rtPA) represented by the lag phase, rate of protofibril formation (Vmax), fibrin structure (ΔAbs), time to peak (TTP), half lysis time (t50 and area under the curve (AUC) were measured in turbidometric clot formation and lysis assays. In the fibrinolysis assay, Vmax was lower in patients than in healthy controls. No differences in the other parameters of clot formation and lysis were detected between the groups. There was no clear association of plasma clot properties with the clinical severity of bleeding in patients with MBDs. Patients with known decreased factor VIII levels also showed a lower Vmax. Fibrinogen levels were positively associated with each of the assessed parameters in both groups, with the strongest association with ΔAbs, indicating altered fibrin structure. Factor VIII activity correlated with altered clot characteristics similar to fibrinogen, especially in patients, with the strongest positive correlation to Vmax. This cohort of patients with MBDs of unknown origin showed a lower rate of fibrin formation in the fibrinolysis assay, but otherwise similar plasma clot properties compared to healthy controls.
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References
Alzahrani SH, Ajjan RA (2010) Coagulation and fibrinolysis in diabetes. Diab Vasc Dis Res 7:260–273. doi:10.1177/1479164110383723
Fatah K, Silveira A, Tornvall P, Karpe F, Blomback M, Hamsten A (1996) Proneness to formation of tight and rigid fibrin gel structures in men with myocardial infarction at a young age. Thromb Haemost 76:535–540
Morris TA, Marsh JJ, Chiles PG, Magana MM, Liang NC, Soler X, Desantis DJ, Ngo D, Woods VL Jr (2009) High prevalence of dysfibrinogenemia among patients with chronic thromboembolic pulmonary hypertension. Blood 114:1929–1936. doi:10.1182/blood-2009-03-208264
Undas A, Nowakowski T, Ciesla-Dul M, Sadowski J (2011) Abnormal plasma fibrin clot characteristics are associated with worse clinical outcome in patients with peripheral arterial disease and thromboangiitis obliterans. Atherosclerosis 215:481–486. doi:10.1016/j.atherosclerosis.2010.12.040
Undas A, Szuldrzynski K, Stepien E, Zalewski J, Godlewski J, Tracz W, Pasowicz M, Zmudka K (2008) Reduced clot permeability and susceptibility to lysis in patients with acute coronary syndrome: effects of inflammation and oxidative stress. Atherosclerosis 196:551–557. doi:S0021-9150(07)00381-4
Undas A, Zawilska K, Ciesla-Dul M, Lehmann-Kopydlowska A, Skubiszak A, Ciepluch K, Tracz W (2009) Altered fibrin clot structure/function in patients with idiopathic venous thromboembolism and in their relatives. Blood 114:4272–4278. doi:blood-2009-010.1182/blood-2009-05-222380
Antovic A, Mikovic D, Elezovic I, Zabczyk M, Hutenby K, Antovic JP (2014) Improvement of fibrin clot structure after factor VIII injection in haemophilia A patients treated on demand. Thromb Haemost 111:656–661. doi:10.1160/TH13-06-0479
Brummel-Ziedins KE, Branda RF, Butenas S, Mann KG (2009) Discordant fibrin formation in hemophilia. J Thromb Haemost 7:825–832. doi:10.1111/j.1538-7836.2009.03306.x
Gray LD, Hussey MA, Larson BM, Machlus KR, Campbell RA, Koch G, Ezban M, Hedner U, Wolberg AS (2011) Recombinant factor VIIa analog NN1731 (V158D/E296V/M298Q-FVIIa) enhances fibrin formation, structure and stability in lipidated hemophilic plasma. Thromb Res 128:570–576. doi:10.1016/j.thromres.2011.04.009
He S, Blomback M, Jacobsson Ekman G, Hedner U (2003) The role of recombinant factor VIIa (FVIIa) in fibrin structure in the absence of FVIII/FIX. J Thromb Haemost 1:1215–1219
Quiroga T, Mezzano D (2012) Is my patient a bleeder? A diagnostic framework for mild bleeding disorders. Hematol Am Soc Hematol Educ Prog 2012:466–474. doi:10.1182/asheducation-2012.1.466
Haselboeck J, Panzer S, Janig F, Kepa S, Mannhalter C, Pabinger I (2013) Characterization of adult patients with a mild to moderate bleeding phenotype from the Vienna Bleeding Biobank. ISTH meeting abstracts No. PB 2.41-4
Ay C, Haselbock J, Laczkovics C, Koder S, Pabinger I (2011) Thrombin generation in patients with a bleeding tendency of unknown origin. Ann Hematol 90:1099–1104. doi:10.1007/s00277-011-1201-8
Rodeghiero F, Castaman G, Tosetto A, Batlle J, Baudo F, Cappelletti A, Casana P, De Bosch N, Eikenboom JC, Federici AB, Lethagen S, Linari S, Srivastava A (2005) The discriminant power of bleeding history for the diagnosis of type 1 von Willebrand disease: an international, multicenter study. J Thromb Haemost 3:2619–2626. doi:10.1111/j.1538-7836.2005.01663.x
Karpati L, Penke B, Katona E, Balogh I, Vamosi G, Muszbek L (2000) A modified, optimized kinetic photometric assay for the determination of blood coagulation factor XIII activity in plasma. Clin Chem 46:1946–1955
Meltzer ME, Lisman T, de Groot PG, Meijers JC, le Cessie S, Doggen CJ, Rosendaal FR (2010) Venous thrombosis risk associated with plasma hypofibrinolysis is explained by elevated plasma levels of TAFI and PAI-1. Blood 116:113–121. doi:10.1182/blood-2010-02-267740
Carr ME Jr, Hermans J (1978) Size and density of fibrin fibers from turbidity. Macromolecules 11:46–50
Scott EM, Ariens RA, Grant PJ (2004) Genetic and environmental determinants of fibrin structure and function: relevance to clinical disease. Arterioscler Thromb Vasc Biol 24:1558–1566. doi:10.1161/01.ATV.0000136649.83297.bf
Blomback B, Carlsson K, Fatah K, Hessel B, Procyk R (1994) Fibrin in human plasma: gel architectures governed by rate and nature of fibrinogen activation. Thromb Res 75:521–538
Scrutton MC, Ross-Murphy SB, Bennett GM, Stirling Y, Meade TW (1994) Changes in clot deformability—a possible explanation for the epidemiological association between plasma fibrinogen concentration and myocardial infarction. Blood Coagul Fibrinolysis 5:7
Blomback B (1994) Fibrinogen structure, activation, polymerization and fibrin gel structure. Thromb Res 75:327–328
Omarova F, Uitte De Willige S, Ariens RA, Rosing J, Bertina RM, Castoldi E (2013) Inhibition of thrombin-mediated factor V activation contributes to the anticoagulant activity of fibrinogen gamma’. J Thromb Haemost 11:1669–1678. doi:10.1111/jth.12354
Alzahrani SH, Hess K, Price JF, Strachan M, Baxter PD, Cubbon R, Phoenix F, Gamlen T, Ariens RA, Grant PJ, Ajjan RA (2012) Gender-specific alterations in fibrin structure function in type 2 diabetes: associations with cardiometabolic and vascular markers. J Clin Endocrinol Metab 97:E2282–E2287. doi:10.1210/jc.2012-2128
Dunn EJ, Ariens RA, Grant PJ (2005) The influence of type 2 diabetes on fibrin structure and function. Diabetologia 48:1198–1206. doi:10.1007/s00125-005-1742-2
Wolberg AS, Allen GA, Monroe DM, Hedner U, Roberts HR, Hoffman M (2005) High dose factor VIIa improves clot structure and stability in a model of haemophilia B. Br J Haematol 131:645–655. doi:10.1111/j.1365-2141.2005.05820.x
Wolberg AS (2010) Plasma and cellular contributions to fibrin network formation, structure and stability. Haemophilia 16(Suppl 3):7–12. doi:10.1111/j.1365-2516.20
Wolberg AS, Campbell RA (2008) Thrombin generation, fibrin clot formation and hemostasis. Transfus Apher Sci 38:15–23. doi:10.1016/j.transci.2007.12.005
Wolberg AS, Monroe DM, Roberts HR, Hoffman M (2003) Elevated prothrombin results in clots with an altered fiber structure: a possible mechanism of the increased thrombotic risk. Blood 101:3008–3013. doi:10.1182/blood-2002-08-2527
Wolberg AS (2007) Thrombin generation and fibrin clot structure. Blood Rev 21:131–142. doi:10.1016/j.blre.2006.11.001
Konings J, Govers-Riemslag JW, Philippou H, Mutch NJ, Borissoff JI, Allan P, Mohan S, Tans G, Ten Cate H, Ariens RA (2011) Factor XIIa regulates the structure of the fibrin clot independently of thrombin generation through direct interaction with fibrin. Blood 118:3942–3951. doi:10.1182/blood-2011-03-339572
Acknowledgments
This study was conducted in collaboration with the MedUni Wien Biobank. J. Gebhart has received financial support of the Bayer Hemophilia Clinical Training Award (http://www.bayer-hemophilia-awards.com). We thank CSL Behring for the financial support of the study by an unrestricted grant.
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The authors declare that they have no competing interests.
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Informed consent was obtained from all individuals (patients and healthy controls) for being included in the study.
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Gebhart, J., Laczkovics, C., Posch, F. et al. Plasma clot properties in patients with a mild-to-moderate bleeding tendency of unknown cause. Ann Hematol 94, 1301–1310 (2015). https://doi.org/10.1007/s00277-015-2399-7
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DOI: https://doi.org/10.1007/s00277-015-2399-7