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Over-representation of bcr3 subtype of PML/RARα fusion gene in APL in Indian patients

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Abstract

Thirty six patients with acute promyelocytic leukemia were studied by reverse transcriptase–polymerase chain reaction (RT-PCR) and real-time PCR. There was concordance between the results achieved by both the methods except in one case, which was negative by RT-PCR but positive by real-time PCR. The prevalence of bcr3 (short isoform) was found to be significantly higher than that of bcr1 (long isoform ) (64 vs. 36%, P=0.03). No correlation was found between age, sex, and white blood cell (WBC) count at diagnosis. Molecular remission was achieved in 66.6% of patients with bcr3 isoform. Median WBC count at presentation was found to be higher than that in the West.

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Acknowledgements

We are grateful to Professional Biotech Pvt. Ltd. for assistance with real-time PCR studies. We are also thankful to Ms. Noor Sabri, Nitin, and Ms. Anshu Khattar for technical assistance.

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Authors and Affiliations

  1. Department of Haematology, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Sudha Sazawal, Syed Khizer Hasan, Pankhi Dutta, Bijender Kumar, Rajat Kumar, V. P. Choudhry & Renu Saxena

  2. Department of Medical Oncology, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Lalit Kumar

Authors
  1. Sudha Sazawal
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  2. Syed Khizer Hasan
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  3. Pankhi Dutta
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  4. Bijender Kumar
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  5. Rajat Kumar
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  6. Lalit Kumar
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  7. V. P. Choudhry
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  8. Renu Saxena
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Correspondence to Renu Saxena.

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Sazawal, S., Hasan, S.K., Dutta, P. et al. Over-representation of bcr3 subtype of PML/RARα fusion gene in APL in Indian patients. Ann Hematol 84, 781–784 (2005). https://doi.org/10.1007/s00277-005-1095-4

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  • DOI: https://doi.org/10.1007/s00277-005-1095-4

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