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Effects of Harvest Sites on Cryopreserved Adipose-Derived Stem Cells and ASC-Enriched Fat Grafts

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  • Basic Science/Experimental
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Abstract

Background

Enrichment of adipose-derived stem cells (ASCs) with fat grafts has demonstrated benefit for graft retention and histologic appearance. There is no consensus on the optimal harvest site for adipose-derived stem cells. This study aimed to investigate the effects of harvest sites on the characteristics of cryopreserved adipose-derived stem cells and the graft retention of cell-assisted lipotransfer.

Methods

Lipoaspirates were harvested from 18 healthy volunteers who underwent liposuctions for body contouring. Twenty milliliters of lipoaspirates was, respectively, obtained from four sites, including the upper limb, abdomen, waist, and thighs, by the Coleman technique. Adipose-derived stem cells were ex vivo cultured and cryopreserved for four weeks. The biological characteristics of ASCs from four harvest sites were analyzed: MSC surface markers, cell proliferation, migration ability, and multipotential differentiation. The fat grafts were co-implanted with ASCs from four harvest sites and injected subcutaneously in mice. The ASC-enriched fat grafts were analyzed three months after transplantation.

Results

Cryopreserved ASCs from the abdomen and thighs maintained more significant cell proliferation, migration ability, and differentiation potential, compared with cells from the upper limb and waist. Moreover, we achieved better graft retention of cell-assisted fat grafts with cryopreserved ASC from the abdomen and thighs.

Conclusions

The harvest site of adipose tissue affects the cellular activity and differentiation potential of cryopreserved ASCs. Improved understanding of harvest sites for ASCs can optimize the outcomes of cell-assisted fat grafts. Fat grafts enriched with cryopreserved ASCs from the abdomen or thighs are the optimal choices.

Level of Evidence V

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Acknowledgements

This article was supported by the Chinese Academy of Medical Sciences Initiative for Innovative Medicine (CAMS-I2M) (2017-I2M-3-006).

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Authors and Affiliations

  1. Breast Plastic and Reconstructive Surgery Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 33 Badachu Road, Shijingshan District, Beijing, 100144, China

    Yaping Qu, Dali Mu, Zifei Li, Tong Liu, Su Fu & Jie Luan

  2. Research Center, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

    Qian Wang

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  1. Yaping Qu
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  2. Dali Mu
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  3. Qian Wang
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  5. Tong Liu
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  6. Su Fu
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  7. Jie Luan
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Correspondence to Jie Luan.

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The authors declared no conflicts of interest concerning the research, authorship, and publication of this article.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all patients.

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Electronic supplementary material

Below is the link to the electronic supplementary material. Supplemental Fig. 1. MSC surface markers of cryopreserved adipose-derived stem cells from four harvest sites. (a) The upper limb group; (b) the abdomen group; (c) the waist group; (d) the thigh group.

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Qu, Y., Mu, D., Wang, Q. et al. Effects of Harvest Sites on Cryopreserved Adipose-Derived Stem Cells and ASC-Enriched Fat Grafts. Aesth Plast Surg 44, 2286–2296 (2020). https://doi.org/10.1007/s00266-020-01900-1

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  • DOI: https://doi.org/10.1007/s00266-020-01900-1

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