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Effects of Platelet-Rich Plasma on Fat and Nanofat Survival: An Experimental Study on Mice

  • Original Article
  • Basic Science/Experimental
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Abstract

Background

Nanofat and fat graft survival is an important clinical problem. The authors of this study investigated whether PRP has an impact on fat and nanofat graft survival and vascularization in a mouse model.

Materials and Methods

Fat was harvested from a 50-year-old healthy woman by vacuum suction, and nanofat was obtained by emulsification and centrifugation procedures. PRP was collected after two rounds of centrifugation from an autologous blood sample. Twenty male nude mice were divided into four treatment groups: PRP/nanofat, PRP/fat, saline/nanofat and saline/fat. After 1 month and 3 months, the grafts were extracted and weighed. The microstructure of the fat and nanofat was examined with a scanning electron microscope. HE and immunohistochemical staining was applied to observe neovascularization. Western blot analysis was used to analyse the expression of CD31 and VEGF.

Results

In fat tissue, fat cells had normal connections; the fat structure was complete and fibre networks were visible. In nanofat, the extracellular matrix vascular components were visible and their structures were intact. At 1 month and 3 months, the graft weights in the PRP/fat group were significantly higher than those in the other groups. Further, a higher degree of neovascularization was observed in the PRP/nanofat group, and the expression of CD31 and VEGF in the PRP/nanofat group was higher than that in the other groups.

Conclusion

PRP can promote nanofat and fat graft survival and vascularization.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Nos. 81171812, 81671924 and 81272105), the National Key Research and Development Plan of China (No. 2017YFC1103301), the National Basic Science and Development Program (No. 2012CB518105), Health and Medical Treatment Collaborative Innovation Major Special Projects of Guangzhou (No. 201508020253), the Science and Technology Program of Guangzhou (201508020115) and Science and Technology Project of Guangdong province (Nos. 2014B020212010, 508113150092, 2015A010101313 and 2017A050506011).

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Authors and Affiliations

  1. The Graduate School of Southern Medical University, Guangzhou, 510515, China

    Xiaoxuan Lei, Huan Liu, Mengru Pang, Zhifang Zheng & Biao Cheng

  2. Center of Wound Treatment, General Hospital of Southern Theater Command, PLA, Guangzhou, 510010, China

    Xiaoxuan Lei, Huan Liu, Mengru Pang, Xi Tan & Biao Cheng

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  1. Xiaoxuan Lei
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  2. Huan Liu
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Correspondence to Biao Cheng.

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Conflict of interest

The authors declare that they have no conflicts of interest in relation to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

Statement of Human and Animal Rights

All the experiments were approved by the Animal Care Committee of Guangzhou General Hospital. All applicable institutional and/or national guidelines for the care and use of animals were followed. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee.

Informed Consent

A 50-year-old healthy woman was selected for abdominal liposuction from the Plastic Surgery Department of the General Hospital. Her signed informed consent was obtained before the procedure was performed.

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Lei, X., Liu, H., Pang, M. et al. Effects of Platelet-Rich Plasma on Fat and Nanofat Survival: An Experimental Study on Mice. Aesth Plast Surg 43, 1085–1094 (2019). https://doi.org/10.1007/s00266-019-01355-z

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