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Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina

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Abstract

Rauvolfia serpentina (L). Benth. ex Kurz. (Apocynaceae), commonly known as Sarpagandha or Indian snakeroot, has long been used in the traditional treatment of snakebites, hypertension, and mental illness. The plant is known to produce an array of indole alkaloids such as reserpine, ajmaline, amalicine, etc. which show immense pharmacological and biomedical significance. However, owing to its poor seed viability, lesser germination rate and overexploitation for several decades for its commercially important bioactive constituents, the plant has become endangered in its natural habitat. The present review comprehensively encompasses the various biotechnological tools employed in this endangered Ayurvedic plant for its in vitro propagation, role of plant growth regulators and additives in direct and indirect regeneration, somatic embryogenesis and synthetic seed production, secondary metabolite production in vitro, and assessment of clonal fidelity using molecular markers and genetic transformation. In addition, elicitation and other methods of optimization of its indole-alkaloids are also described herewith.

Key points

• Latest literature on in vitro propagation of Rauvolfia serpentina

• Biotechnological production and optimization of indole alkaloids

• Clonal fidelity and transgenic studies in R. serpentina

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Author notes

  1. Abhijit Dey and Debleena Roy have an equal contribution.

Authors and Affiliations

  1. Department of Life Sciences, Presidency University, 86/1 College Street, Kolkata, 700073, West Bengal, India

    Abhijit Dey & Mimosa Ghorai

  2. PG Department of Botany, Lady Brabourne College, Kolkata, West Bengal, India

    Debleena Roy

  3. Department of Botany, Rashtrapita Mahatma Gandhi Arts and Science College, Nagbhid, Maharashtra, India, 441205

    Vikas Moreshwar Mohture

  4. Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Gangwon-do, Wonju, 26426, Korea

    Md. Habibur Rahman

  5. Department of Life Sciences, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel

    Uttpal Anand

  6. Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700 032, West Bengal, India

    Saikat Dewanjee

  7. School of Biological and Environmental Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, Himachal Pradesh, India

    Radha

  8. Chemical and Biochemical Processing Division, ICAR - Central Institute for Research On Cotton Technology, Mumbai, 400019, India

    Manoj Kumar

  9. Department of Microbiology, School of Biosciences, Periyar University, Salem, 636011, Tamilnadu, India

    Dorairaj Arvind Prasanth

  10. Department of Biotechnology, School of Engineering & Technology (SET), Sharda University, Greater Noida 201310, Uttar Pradesh, India

    Niraj Kumar Jha & Saurabh Kumar Jha

  11. Department of Biotechnology Engineering and Food Technology, Chandigarh University, Mohali, 140413, India

    Saurabh Kumar Jha

  12. Department of Biotechnology, School of Applied & Life Sciences (SALS), Uttaranchal University, Dehradun, 248007, India

    Saurabh Kumar Jha

  13. Plant Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, 605 008, India

    Mahipal S. Shekhawat

  14. Department of Biotechnology, Faculty of Technology and Sciences, Lovely Professional University, Phagwara, Punjab, India

    Devendra Kumar Pandey

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  1. Abhijit Dey
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  3. Vikas Moreshwar Mohture
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  4. Mimosa Ghorai
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Contributions

AD, DR, VMM, and MG conceptualized designed review. MHR, UA, and SD prepared the primary draft. R and MK conducted the literature survey. DAP, NKJ, and SKJ contributed figures or analytical tools. MSS and DKP analyzed and finalized the manuscript. All authors read and approved the manuscript.

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Correspondence to Abhijit Dey or Devendra Kumar Pandey.

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Dey, A., Roy, D., Mohture, V.M. et al. Biotechnological interventions and indole alkaloid production in Rauvolfia serpentina. Appl Microbiol Biotechnol 106, 4867–4883 (2022). https://doi.org/10.1007/s00253-022-12040-8

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  • DOI: https://doi.org/10.1007/s00253-022-12040-8

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