Abstract
A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora. For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C. After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility. Furthermore, high degradation of Miscanthus structural carbohydrates occurred during the first generation. A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed. Additionally, changes in the bacterial community occurred that might be associated with unwanted cellulose degradation in the system. This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
Key points
• Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
• Feedstock microbial reduction is necessary for pretreatment with C. subvermispora.
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Availability of data and material
The datasets generated during the current study are available in the Sequence Read Archive (SRA) repository, accession PRJNA756393. All other data generated during the current study are available from the corresponding author on reasonable request.
Code availability
Not applicable.
References
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Acknowledgements
The authors acknowledge the assistance and helpful suggestions of Dr. Michael Joe Vaughan and Dr. Fiorella Cisneros-Carter.
Funding
This work was supported in part by the USDA National Institute of Food and Agriculture Federal Appropriations under Project PEN04671 and Accession number 1017582, and award number 2012–10008-20302. Funding was also received for Dr. Vasco-Correa by the Ohio Agricultural Research and Development Center, Minciencias Colombia, and Fulbright Colombia.
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J.V. and T.M. conceived and designed research. J.V. conducted experiments. R.C. performed the bioinformatic analyses. J.V. and R.C. analyzed data. J.V. and R.C. wrote the manuscript. A.S. and T.M. supervised the work. All authors read and approved the manuscript.
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Vasco-Correa, J., Capouya, R., Shah, A. et al. Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities. Appl Microbiol Biotechnol 106, 2263–2279 (2022). https://doi.org/10.1007/s00253-022-11833-1
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DOI: https://doi.org/10.1007/s00253-022-11833-1