Abstract
The general public spends one-third of its time under artificial lighting, which lacks bands beneficial to human health, and long-term exposure will have a negative impact on bone health. Here, we report the effects of long-term, low-dose ultraviolet (UV) supplementation to white light-emitting diode (LED) light exposure on intestinal microorganisms and bone metabolism, as well as the correlations between the two. Normal and ovariectomized rats were irradiated with LED white light with or without supplementation with UV. The effects of UV supplementation on the intestinal flora and the relationship between the intestinal flora and bone were investigated by measuring the intestinal flora, bone metabolism markers, and bone histomorphology. UV supplementation affected the bone density and bone mass by changing the relative content of Firmicutes, Saccharibacteria, and Proteobacteria; however, the intestinal flora were not the only factors affecting bone. Ultraviolet supplementation changed the composition and function of the gut flora in the bone loss model. By increasing the synthesis of short-chain fatty acids and affecting immunomodulatory, intestinal flora directly or indirectly regulate the activity of osteoclasts and thus mediate UV-mediated improvements in bone metabolism. Our work shows that UV supplementation affects bone density by influencing the intestinal flora, introducing a novel strategy to develop healthier artificial light sources and prevent bone loss.
Key points
• We measured the bone metabolism markers and bone histomorphometry of rats.
• The diversity, composition, and function of intestinal flora were analyzed.
• The relationship between gut microbiota and host bone physiology was analyzed.
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Acknowledgements
This work was financially supported by grants from the National Natural Science Foundation of China (81871520, 31870852) and the Foundation for the Excellent Youth Scholars of Shandong Educational Committee (72).
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The data that support the findings of this study are openly available in the NCBI SRA repository, reference number PRJNA671564.
Funding
This work was financially supported by grants from the National Natural Science Foundation of China (81871520, 31870852) and the Foundation for the Excellent Youth Scholars of Shandong Educational Committee (72).
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JC and YF conceived and designed research. JC conducted experiments. JC, ZY, and CD analyzed data. YF and HL guided most experiments. JC and YF wrote the manuscript. All authors read and approved the manuscript.
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Cui, J., Fu, Y., Yi, Z. et al. The beneficial effects of ultraviolet light supplementation on bone density are associated with the intestinal flora in rats. Appl Microbiol Biotechnol 105, 3705–3715 (2021). https://doi.org/10.1007/s00253-021-11282-2
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DOI: https://doi.org/10.1007/s00253-021-11282-2