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Biostimulation of calcite precipitation process by bacterial community in improving cement stabilized rammed earth as sustainable material

  • Environmental biotechnology
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Abstract

Rammed earth has been enjoying a renaissance as sustainable construction material with cement stabilized rammed earth (CSRE). At the same time, it is important to convert CSRE to be a stronger, durable, and environment-friendly building material. Bacterial application is established to improve cementitious materials; however, bioaugmentation is not widely acceptable by engineering communities. Hence, the present study is an attempt applying biostimulation approach to develop CSRE as sustainable construction material. Results showed that biostimulation improved the compressive strength of CSRE by 29.6% and resulted in 27.7% lower water absorption compared to control. The process leading to biocementation in improving CSRE was characterized by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscope-energy dispersive spectrometer. Further, Illumina MiSeq sequencing was used to investigate changes in bacterial community structures after biostimulation that identified majority of ureolytic bacteria dominated by phylum Firmicutes and genus Sporosarcina playing role in biocementation. The results open a way applying biological principle that will be acceptable to a wide range of civil engineers.

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References

  • Abo-El-Enein SA, Ali AH, Talkhan FN, Abdel-Gawwad HA (2013) Application of microbial biocementation to improve the physico-mechanical properties of cement mortar. HBRC J 9:36–40

    Article  Google Scholar 

  • Achal V, Mukherjee A, Basu PC, Reddy MS (2009) Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J Ind Microbiol Biotechnol 36:433–438

    Article  CAS  PubMed  Google Scholar 

  • Chhaiba S, Blanco-Varela MT, Diouri A, Bougarrani S (2018) Characterization and hydration of cements and pastes obtained from raw mix containing Moroccan oil shale and coal waste as a raw material. Constr Build Mater 189:539–549

    Article  CAS  Google Scholar 

  • Cuzman OA, Rescic S, Richter K, Wittig L, Tiano P (2015) Sporosarcina pasteurii use in extreme alkaline conditions for recycling solid industrial wastes. J Biotechnol 214:49–56

    Article  CAS  PubMed  Google Scholar 

  • Duo L, Kan-liang T, Hui-li Z, Yu-yao W, Kang-yi N, Shi-can Z (2018) Experimental investigation of solidifying desert aeolian sand using microbially induced calcite precipitation. Constr Build Mater 172:251–262

    Article  CAS  Google Scholar 

  • Fisher KA, Yarwood SA, James BR (2017) Soil urease activity and bacterial ureC gene copy numbers: effect of pH. Geoderma 285:1–8

    Article  CAS  Google Scholar 

  • François B, Palazon L, Gerard P (2017) Structural behaviour of unstabilized rammed earth constructions submitted to hygroscopic conditions. Constr Build Mater 155:164–175

    Article  Google Scholar 

  • Girão AV, Richardson IG, Porteneuve CB, Brydson RMD (2007) Composition, morphology and nanostructure of C–S–H in white Portland cement pastes hydrated at 55 °C. Cem Concr Res 37:1571–1582

    Article  CAS  Google Scholar 

  • Hamady M, Lozupone C, Knight R (2010) Fast UniFrac: facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data. ISME J 4:17–27

    Article  CAS  PubMed  Google Scholar 

  • Hamm AC, Tenuta M, Krause DO, Ominski KH, Tkachuk VL, Flaten DN (2016) Bacterial communities of an agricultural soil amended with solid pig and dairy manures, and urea fertilizer. Appl Soil Ecol 103:61–71

    Article  Google Scholar 

  • Jiang J, Zheng Q, Yan Y, Guo D, Wang F, Wu S, Sun W (2018) Design of a novel nanocomposite with C-S-H@LA for thermal energy storage: a theoretical and experimental study. Appl Energy 220:395–407

    Article  CAS  Google Scholar 

  • Kanchanason V, Plank J (2017) Role of pH on the structure, composition and morphology of C-S-H–PCE nanocomposites and their effect on early strength development of Portland cement. Cem Concr Res 102:90–98

    Article  CAS  Google Scholar 

  • Kariyawasam KKGKD, Jayasinghe C (2016) Cement stabilized rammed earth as a sustainable construction material. Constr Build Mater 105:519–527

    Article  Google Scholar 

  • Kumari D, Pan XL, Lee DJ, Achal V (2014) Immobilization of cadmium in soil by microbially induced carbonate precipitation with Exiguobacterium undae at low temperature. Int Biodeterior Biodegradation 94:98–102

    Article  CAS  Google Scholar 

  • Kunchariyakun K, Asavapisit S, Sinyoung S (2018) Influence of partial sand replacement by black rice husk ash and bagasse ash on properties of autoclaved aerated concrete under different temperatures and times. Constr Build Mater 173:220–227

    Article  CAS  Google Scholar 

  • Lazzari E, Schena T, Marcelo MCA, Primaz CT, Silva AN, Ferrão MF, Bjerk T, Caramão EB (2018) Classification of biomass through their pyrolytic bio-oil composition using FTIR and PCA analysis. Ind Crop Prod 111:856–864

    Article  CAS  Google Scholar 

  • Liu W, Cai W, Guo Z, Wang L, Yang C, Varrone C, Wang A (2016) Microbial electrolysis contribution to anaerobic digestion of waste activated sludge, leading to accelerated methane production. Renewable Ener 91:334–339

    Article  CAS  Google Scholar 

  • Qian XY, Fang C, Huang M, Achal V (2017) Characterization of fungal-mediated carbonate precipitation in the biomineralization of chromate and lead from an aqueous solution and soil. J Clean Prod 164:198–208

    Article  CAS  Google Scholar 

  • Qing-ru Z, Bo-han L, Li-tian Z, Xi-hong Z, Hong-xiao T (2006) Short-term alleviation of aluminum phytotoxicity by urea application in acid soils from South China. Chemosphere 63:860–868

    Article  CAS  PubMed  Google Scholar 

  • Ramli MB, Alonge OR (2016) Characterization of metakaolin and study on early age mechanical strength of hybrid cementitious composites. Constr Build Mater 121:599–611

    Article  CAS  Google Scholar 

  • Reddy BVV, Kumar PP (2010) Embodied energy in cement stabilised rammed earth walls. Energy Build 42:380–385

    Article  Google Scholar 

  • Seifan M, Samani AK, Berenjian A (2016) Induced calcium carbonate precipitation using Bacillus species. Appl Microbiol Biotechnol 100:9895–9906

    Article  CAS  PubMed  Google Scholar 

  • Sekhar DC, Nayak S (2018) Utilization of granulated blast furnace slag and cement in the manufacture of compressed stabilized earth blocks. Constr Build Mater 166:531–536

    Article  Google Scholar 

  • Sharma LK, Sirdesai NN, Sharma KM, Singh TN (2018) Experimental study to examine the independent roles of lime and cement on the stabilization of a mountain soil: a comparative study. Appl Clay Sci 152:183–195

    Article  CAS  Google Scholar 

  • Siddiqua S, Barreto PNM (2018) Chemical stabilization of rammed earth using calcium carbide residue and fly ash. Constr Build Mater 169:364–371

    Article  CAS  Google Scholar 

  • Silva RA, Oliveira DV, Miranda T, Cristelo N, Escobar MC, Soares E (2013) Rammed earth construction with granitic residual soils: the case study of northern Portugal. Constr Build Mater 47:181–191

    Article  Google Scholar 

  • Sun J, Shi H, Qian B, Xu Z, Li W, Shen X (2017) Effects of synthetic C-S-H/PCE nanocomposites on early cement hydration. Constr Build Mater 140:282–292

    Article  CAS  Google Scholar 

  • Sun S, Lin J, Zhang P, Fang L, Ma R, Quan Z, Song X (2018) Geopolymer synthetized from sludge residue pretreated by the wet alkalinizing method: compressive strength and immobilization efficiency of heavy metal. Constr Build Mater 170:619–626

    Article  CAS  Google Scholar 

  • Tripura DD, Singh KD (2015) Axial load-capacity of rectangular cement stabilized rammed earth column. Eng Struct 99:402–412

    Article  Google Scholar 

  • Xu J, Wang X, Wang B (2018) Biochemical process of ureolysis-based microbial CaCO3 precipitation and its application in self-healing concrete. Appl Microbiol Biotechnol 102:3121–3132

  • Yang H, Monasterio M, Cui H, Han N (2017) Experimental study of the effects of graphene oxide on microstructure and properties of cement paste composite. Composites Part A: Appl Sci Manufac 102:263–272

    Article  CAS  Google Scholar 

  • Yang Z, Xu X, Dai M, Wang L, Shi X, Guo R (2018) Combination of bioaugmentation and biostimulation for remediation of paddy soil contaminated with 2,4-dichlorophenoxyacetic acid. J Hazard Mater 353:490–495

    Article  CAS  PubMed  Google Scholar 

  • Yuan Q-B, Shen Y, Huang Y-M, Hu N (2018) A comparative study of aeration, biostimulation and bioaugmentation in contaminated urban river purification. Environ Technol Innov 11:276–285

    Article  Google Scholar 

  • Zhu X, Kumari D, Huang MS, Achal V (2016a) Biosynthesis of CdS nanoparticles through microbial induced calcite precipitation. Mater Design 98:209–214

  • Zhu X, Li W, Zhan L, Huang MS, Zhang Q, Achal V (2016b) The large-scale process of microbial carbonate precipitation for nickel remediation from an industrial soil. Environ Pollut 219:149–155

    Article  CAS  PubMed  Google Scholar 

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  1. Environmental Engineering Program, Guangdong Technion – Israel Institute of Technology, Shantou, 515063, China

    Chaolin Fang & Varenyam Achal

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  1. Chaolin Fang
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  2. Varenyam Achal
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Correspondence to Varenyam Achal.

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Fang, C., Achal, V. Biostimulation of calcite precipitation process by bacterial community in improving cement stabilized rammed earth as sustainable material. Appl Microbiol Biotechnol 103, 7719–7727 (2019). https://doi.org/10.1007/s00253-019-10024-9

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  • DOI: https://doi.org/10.1007/s00253-019-10024-9

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