Abstract
Oxidative stress plays a detrimental role in gastrointestinal disorders. Although selenium-enriched probiotics have been shown to strengthen oxidation resistance and innate immunity, the potential mechanism remains unclear. Here, we focused on the biological function of our material, selenium-enriched Bacillus paralicheniformis SR14 (Se-BP), and investigated the antioxidative effects of Se-BP and its underlying molecular mechanism in porcine jejunum epithelial cells. First, we prepared Se-BP and quantified for its selenium and bacterial contents. Then, in vitro free radical scavenging activity was measured to evaluate the potential antioxidant effect of Se-BP. Third, to induce an appropriate oxidative stress model, we adopted different concentrations of H2O2 and determined the most suitable concentration by a methyl thiazolyl tetrazolium (MTT) assay. Regarding treatment with Se-BP and H2O2, we found that Se-BP increased cell viability and prevented lactate dehydrogenase release when administered prior to H2O2 exposure. Additionally, Se-BP markedly suppressed reactive oxygen species and malondialdehyde production in cells and effectively attenuated apoptosis. Compared with incubation with H2O2 alone, treatment with Se-BP significantly promoted phosphorylation of ERK and p38 MAPK signaling molecules. When administered with ERK and p38 MAPK inhibitors, Se-BP did not alleviate the decrease in cell viability. Our results suggest that Se-BP prevents H2O2-induced cell damage by activating the ERK/p38 MAPK signaling pathways.
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Acknowledgments
We thank the staff of the Electronic Microscopy Center and the Agricultural, Biological, and Environmental Test Center at Zhejiang University for their assistance with flow cytometry. We thank Prof. Yulong Yin for the gift of the IPEC-J2 cells.
Funding
This work was supported by grants from the Modern Agroindustry Technology Research System (No. CARS-35) and Zhejiang Province Key R & D Project (No. 2015C02022).
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Xiao, X., Cheng, Y., Song, D. et al. Selenium-enriched Bacillus paralicheniformis SR14 attenuates H2O2-induced oxidative damage in porcine jejunum epithelial cells via the MAPK pathway. Appl Microbiol Biotechnol 103, 6231–6243 (2019). https://doi.org/10.1007/s00253-019-09922-9
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DOI: https://doi.org/10.1007/s00253-019-09922-9