Abstract
Acetic acid bacteria (AAB) are widely used in acetic acid fermentation due to their remarkable ability to oxidize ethanol and high tolerance against acetic acid. In Acetobacter pasteurianus, nucleotide excision repair protein UvrA was up-regulated 2.1 times by acetic acid when compared with that without acetic acid. To study the effects of UvrA on A. pasteurianus acetic acid tolerance, uvrA knockout strain AC2005-ΔuvrA, uvrA overexpression strain AC2005 (pMV24-uvrA), and the control strain AC2005 (pMV24), were constructed. One percent initial acetic acid was almost lethal to AC2005-ΔuvrA. However, the biomass of the UvrA overexpression strain was higher than that of the control under acetic acid concentrations. After 6% acetic acid shock for 20 and 40 min, the survival ratios of AC2005 (pMV24-uvrA) were 2 and 0.12%, respectively; however, they were 1.5 and 0.06% for the control strain AC2005 (pMV24). UvrA overexpression enhanced the acetification rate by 21.7% when compared with the control. The enzymes involved in ethanol oxidation and acetic acid tolerance were up-regulated during acetic acid fermentation due to the overexpression of UvrA. Therefore, in A. pasteurianus, UvrA could be induced by acetic acid and is related with the acetic acid tolerance by protecting the genome against acetic acid to ensure the protein expression and metabolism.
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Acknowledgements
The authors would like to acknowledge the Mizkan Group Corporation, Japan, for their plasmid pMV24 and Dr. Wei Liujing (East China University of Science and Technology, China) for gifting the plasmids pSUP202 and pRK2013.
Funding
This work was supported by the National Natural Science Foundation of China (31201406, 31671851), Tianjin Municipal Science and Technology Commission (16YFZCNC00650, 17PTGCCX00190), Rural Affairs Committee of Tianjin (201701180), Program for Changjiang Scholars and the Innovative University Research Team (IRT15R49), and the Innovative Research Team of Tianjin Municipal Education Commission (TD13-5013).
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Zheng, Y., Wang, J., Bai, X. et al. Improving the acetic acid tolerance and fermentation of Acetobacter pasteurianus by nucleotide excision repair protein UvrA. Appl Microbiol Biotechnol 102, 6493–6502 (2018). https://doi.org/10.1007/s00253-018-9066-6
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DOI: https://doi.org/10.1007/s00253-018-9066-6