Abstract
Actinobacteria are extremely important to human health, agriculture, and forests. Because of the vast differences of the characteristics of Actinobacteria, a lot of genetic tools have been developed for efficiently manipulating the genetics. Although there are a lot of successful examples of engineering Actinobacteria, they are still more difficult to be genetically manipulated than other model microorganisms such as Saccharomyces cerevisiae, Escherichia coli, and Bacillus subtilis etc. due to the diverse genomics and biochemical machinery. Here, we review the methods to introduce heterologous DNA into Actinobacteria and the available genetic modification tools. The trends and problems existing in engineering Actinobacteria are also covered.
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This study was funded by the National Natural Science Foundation of China (grant number 31500070, 31600044) and the Natural Science Foundation of Jiangsu Province (BK20150136, BK20150151).
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Deng, Y., Zhang, X. & Zhang, X. Recent advances in genetic modification systems for Actinobacteria. Appl Microbiol Biotechnol 101, 2217–2226 (2017). https://doi.org/10.1007/s00253-017-8156-1
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DOI: https://doi.org/10.1007/s00253-017-8156-1