Skip to main content
SpringerLink
Log in

Production of optically pure d-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum

  • Biotechnological products and process engineering
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Simultaneous saccharification and fermentation (SSF) of d-lactic acid was performed using brown rice as both a substrate and a nutrient source. An engineered Lactobacillus plantarum NCIMB 8826 strain, in which the ʟ-lactate dehydrogenase gene was disrupted, produced 97.7 g/L d-lactic acid from 20% (w/v) brown rice without any nutrient supplementation. However, a significant amount of glucose remained unconsumed and the yield of lactic acid was as low as 0.75 (g/g-glucose contained in brown rice). Interestingly, the glucose consumption was significantly improved by adapting L. plantarum cells to the low-pH condition during the early stage of SSF (8–17 h). As a result, 117.1 g/L d-lactic acid was produced with a high yield of 0.93 and an optical purity of 99.6% after 144 h of fermentation. SSF experiments were repeatedly performed for ten times and d-lactic acid was stably produced using recycled cells (118.4–129.8 g/L). On average, d-lactic acid was produced with a volumetric productivity of 2.18 g/L/h over 48 h.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.

Similar content being viewed by others

References

  • Baldwin K, Childs N, Dyck J, Hansen J (2012) Southeast Asia’s rice surplus. A report from the economic research service. United States Department of Agriculture

  • Bruinenberg P, Vos P, de Vos WM (1992) Proteinase overproduction in Lactococcus lactis strains: regulation and effect on growth and acidification in milk. Appl Environ Microbiol 58:78–84

    CAS  PubMed  PubMed Central  Google Scholar 

  • Council for Science and Technology (2015) Standard tables of food composition in Japan 7th ed. Ministry of Education, Culture, Sports, Science and Technology, Japan. National Printing Bureau, Tokyo, Japan

  • Dumbrepatil A, Adsul M, Chaudhari S, Khire J, Gokhale D (2008) Utilization of molasses sugar for lactic acid production by Lactobacillus delbrueckii subsp. delbrueckii mutant Uc-3 in batch fermentation. Appl Environ Microbiol 74:333–335

    Article  CAS  PubMed  Google Scholar 

  • Fukushima K, Sogo K, Miura S, Kimura Y (2004) Production of d-lactic acid by bacterial fermentation of rice starch. Macromol Biosci 4:1021–1027

    Article  CAS  PubMed  Google Scholar 

  • Gao MT, Kaneko M, Hirata M, Toorisaka E, Hano T (2008) Utilization of rice bran as nutrient source for fermentative lactic acid production. Bioresour Technol 99:3659–3664

    Article  CAS  PubMed  Google Scholar 

  • Hama S, Mizuno S, Kihara M, Tanaka T, Ogino C, Noda H, Kondo A (2015) Production of d-lactic acid from hardwood pulp by mechanical milling followed by simultaneous saccharification and fermentation using metabolically engineered Lactobacillus plantarum. Bioresour Technol 187:167–172

    Article  CAS  PubMed  Google Scholar 

  • Ikada Y, Jamshidi K, Tsuji H, Hyon SH (1987) Stereocomplex formation between enantiomeric poly (lactides). Macromolecules 20:904–906

    Article  CAS  Google Scholar 

  • Linko YY, Javanainen P (1996) Simultaneous liquefaction, saccharification, and lactic acid fermentation on barley starch. Enzym Microb Technol 19:118–123

    Article  CAS  Google Scholar 

  • Lunt J (1998) Large-scale production, properties and commercial applications of polylactic acid polymers. Polym Degrad Stabil 59:145–152

    Article  CAS  Google Scholar 

  • Oh H, Wee YJ, Yun JS, Han SH, Jung S, Ryu HW (2005) Lactic acid production from agricultural resources as cheap raw materials. Bioresour Technol 96:1492–1498

    Article  CAS  PubMed  Google Scholar 

  • Ohara H, Owaki M, Sonomoto K (2007) Calculation of metabolic flow of xylose in Lactococcus lactis. J Biosci Bioeng 103:92–94

    Article  CAS  PubMed  Google Scholar 

  • Okano K, Tanaka T, Ogino C, Fukuda H, Kondo A (2010) Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limit. Appl Microbiol Biotechnol 85:413–423

    Article  CAS  PubMed  Google Scholar 

  • Okano K, Zhang Q, Shinkawa S, Yoshida S, Tanaka T, Fukuda H, Kondo A (2009) Efficient production of optically pure d-lactic acid from raw corn starch using genetically modified ʟ-lactate dehydrogenase gene-deficient and α-amylase-secreting Lactobacillus plantarum. Appl Environ Microbiol 75:462–467

    Article  CAS  PubMed  Google Scholar 

  • Pavan S, Hols P, Delcour J, Geoffroy MC, Grangette C, Kleerebezem M, Mercenier A (2000) Adaptation of the nisin-controlled expression system in Lactobacillus plantarum: a tool to study in vivo biological effects. Appl Environ Microbiol 66:4427–4432

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rud I, Jensen PR, Naterstad K, Axelsson L (2006) A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum. Microbiology 152:1011–1019

    Article  CAS  PubMed  Google Scholar 

  • Saman P, Fuciños P, Vázquez JA, Pandiella SS (2011) Fermentability of brown rice and rice bran for growth of human Lactobacillus plantarum NCIMB 8826. Food Technol Biotechnol 49:128–132

    CAS  Google Scholar 

  • Silva J, Carvalho AS, Ferreira R, Vitorino R, Amado F, Domingues P, Teixeira P, Gibbs PA (2005) Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying. J Appl Microbiol 98:775–782

    Article  CAS  PubMed  Google Scholar 

  • Siragusa S, De Angelis M, Calasso M, Campanella D, Minervini F, Di Cagno R, Gobbetti M (2014) Fermentation and proteome profiles of Lactobacillus plantarum strains during growth under food-like conditions. J Proteome 96:366–380

    Article  CAS  Google Scholar 

  • Tanaka T, Hoshina M, Tanabe S, Sakai K, Ohtsubo S, Taniguchi M (2006) Production of d-lactic acid from defatted rice bran by simultaneous saccharification and fermentation. Bioresour Technol 97:211–217

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Yang Z, Qin P, Tan T (2014) Fermentative ʟ-(+)-lactic acid production from defatted rice bran. RSC Adv 4:8907–8913

    Article  CAS  Google Scholar 

  • Wee YJ, Kim JN, Ryu HW (2006) Biotechnological production of lactic acid and its recent applications. Food Technol Biotechnol 44:163–172

    CAS  Google Scholar 

  • Wegkamp A, Teusink B, de Vos WM, Smid EJ (2010) Development of a minimal growth medium for Lactobacillus plantarum. Lett Appl Microbiol 50:57–64

    Article  CAS  PubMed  Google Scholar 

  • Yang P, Wang J, Qi Q (2015) Prophage recombinases-mediated genome engineering in Lactobacillus plantarum. Microb Cell Factories 14:154

    Article  Google Scholar 

  • Yun JS, Wee YJ, Kim JN, Ryu HW (2004) Fermentative production of dʟ-lactic acid from amylase-treated rice and wheat brans hydrolyzate by a novel lactic acid bacterium, Lactobacillus sp. Biotechnol Lett 26:1613–1616

    Article  CAS  PubMed  Google Scholar 

  • Zhang Y, Vadlani PV, Kumar A, Hardwidge PR, Govind R, Tanaka T, Kondo A (2016) Enhance d-lactic acid production from renewable resources using engineered Lactobacillus plantarum. Appl Microbiol Biotechnol 100:279–288

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka, 565-0871, Japan

    Kenji Okano

  2. Bio-energy Corporation, Research & Development Laboratory, 2-9-7 Minaminanamatsu, Amagasaki, 660-0053, Japan

    Shinji Hama, Maki Kihara & Hideo Noda

  3. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan

    Tsutomu Tanaka

  4. Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan

    Akihiko Kondo

Authors
  1. Kenji Okano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shinji Hama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maki Kihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hideo Noda
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tsutomu Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Akihiko Kondo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Akihiko Kondo.

Ethics declarations

Funding

This work was partly financed by the Strategic Foundational Technology Improvement Support Operation of the Kinki Bureau of Economy, Trade and Industry, Japan. We acknowledge Special Coordination Funds for Promoting Science and Technology, Creation of Innovative Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We also thank the Japan Society for the Promotion of Science (JSPS), KAKENHI Grant (16K18299).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Okano, K., Hama, S., Kihara, M. et al. Production of optically pure d-lactic acid from brown rice using metabolically engineered Lactobacillus plantarum . Appl Microbiol Biotechnol 101, 1869–1875 (2017). https://doi.org/10.1007/s00253-016-7976-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-016-7976-8

Keywords

Search

Navigation