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High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater

  • Environmental biotechnology
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A Correction to this article was published on 19 February 2020

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Abstract

In this study, the microbial community structure was assessed in an anaerobic ammonium oxidation–upflow anaerobic sludge blanket (ANAMMOX-UASB) reactor treating high-strength wastewater (approximately 700 mg N L−1 in total nitrogen) by employing Illumina high-throughput sequencing analysis. The reactor was started up and reached a steady state in 26 days by seeding mature ANAMMOX granules, and a high nitrogen removal rate (NRR) of 2.96 kg N m−3 day−1 was obtained at 13.2∼17.6 °C. Results revealed that the abundance of ANAMMOX bacteria increased during the operation, though it occupied a low proportion in the system. The phylum Planctomycetes was only 8.39 % on day 148 and Candidatus Brocadia was identified as the dominant ANAMMOX species with a percentage of 2.70 %. The phylum of Chloroflexi, Bacteroidetes, and Proteobacteria constituted a percentage up to 70 % in the community, of which the Chloroflexi and Bacteroidetes were likely to be related to the sludge granulation. In addition, it was found that heterotrophic denitrifying bacteria of Denitratisoma belonging to Proteobacteria phylum occupied a large proportion (22.1∼23.58 %), which was likely caused by the bacteria lysis and decay with the internal carbon source production. The SEM images also showed that plenty of other microorganisms existed in the ANAMMOX-UASB reactor.

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Change history

  • 19 February 2020

    The published online version contains mistake in the affiliation ID of the author Baikun Li. The correct presentation is given above.

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Acknowledgments

This research was financially supported by Natural Science Foundation of China (21177005) and open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (QAK201502).

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Authors and Affiliations

  1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China

    Shenbin Cao, Nanqi Ren & Yongzhen Peng

  2. Key Laboratory of Beijing for Water Quality Science and Water Environment Recovory Engineering, Engineering Research Center of Beijing, Beijing University of Technology, Beijing, 100124, China

    Rui Du, Baikun Li & Yongzhen Peng

  3. Department of Civil and Environmental Engineering, University of Connecticut, Storrs, CT, 06269, USA

    Baikun Li

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  1. Shenbin Cao
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  2. Rui Du
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  3. Baikun Li
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Correspondence to Yongzhen Peng.

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All of the authors declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.

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Cao, S., Du, R., Li, B. et al. High-throughput profiling of microbial community structures in an ANAMMOX-UASB reactor treating high-strength wastewater. Appl Microbiol Biotechnol 100, 6457–6467 (2016). https://doi.org/10.1007/s00253-016-7427-6

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