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Sugarcane bagasse as support for immobilization of Bacillus pumilus HZ-2 and its use in bioremediation of mesotrione-contaminated soils

  • Environmental biotechnology
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Abstract

The degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. In the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. A novel isolate Bacillus pumilus HZ-2 was applied in bacterial immobilization, which was capable of degrading over 95 % of mesotrione at initial concentrations ranging from 25 to 200 mg L−1 within 4 days in flask-shaking tests. Scanning electron microscope (SEM) images showed that the bacterial cells were strongly absorbed and fully dispersed on bagasse surface after immobilization. Specially, 86.5 and 82.9 % of mesotrione was eliminated by bacteria immobilized on bagasse of 100 and 60 mesh, respectively, which indicated that this immobilization was able to maintain a high degrading activity of the bacteria. Analysis of the degradation products determined 2-amino-4-methylsulfonylbenzoic acid (AMBA) and 4-methylsulfonyl-2-nitrobenzoic acid (MNBA) as the main metabolites in the biodegradation pathway of mesotrione. In the sterile soil, approximately 90 % of mesotrione was degraded after supplementing 5.0 % of molasses in bacteria-bagasse composite, which greatly enhanced microbial adaptability and growth in the soil environment. In the field tests, over 75 % of mesotrione in soil was degraded within 14 days. The immobilized preparation demonstrated that mesotrione could be degraded at a wide range of pH values (5.0–8.0) and temperatures (25–35 °C), especially at low concentrations of mesotrione (5 to 20 mg kg−1). These results showed that sugarcane bagasse might be a good candidate as bacteria-immobilizing support to enhance mesotrione degradation by Bacillus p. HZ-2 in contaminated soils.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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This study was funded by the National Natural Science Foundation of China (grant number 31371960).

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Authors and Affiliations

  1. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, and Lab of Insect Toxicology, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Jie Liu, Jie Ding, Ying Xiao, Haitao Han & Guohua Zhong

  2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Shaohua Chen

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  1. Jie Liu
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  2. Shaohua Chen
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  3. Jie Ding
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  4. Ying Xiao
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  5. Haitao Han
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Correspondence to Guohua Zhong.

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Liu, J., Chen, S., Ding, J. et al. Sugarcane bagasse as support for immobilization of Bacillus pumilus HZ-2 and its use in bioremediation of mesotrione-contaminated soils. Appl Microbiol Biotechnol 99, 10839–10851 (2015). https://doi.org/10.1007/s00253-015-6935-0

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