Abstract
A gene in Bradyrhizobium japonicum USDA 110, annotated as a ribitol dehydrogenase (RDH), had 87 % sequence identity (97 % positives) to the N-terminal 31 amino acids of an l-glucitol dehydrogenase from Stenotrophomonas maltophilia DSMZ 14322. The 729-bp long RDH gene coded for a protein consisting of 242 amino acids with a molecular mass of 26.1 kDa. The heterologously expressed protein not only exhibited the main enantio selective activity with d-glucitol oxidation to d-fructose but also converted l-glucitol to d-sorbose with enzymatic cofactor regeneration and a yield of 90 %. The temperature stability and the apparent K m value for l-glucitol oxidation let the enzyme appear as a promising subject for further improvement by enzyme evolution. We propose to rename the enzyme from the annotated RDH gene (locus tag bll6662) from B. japonicum USDA as a d-sorbitol dehydrogenase (EC 1.1.1.14).
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Acknowledgments
Financial support from the European Community through the FP7-NMP-2007-SMALL 1 collaborative project ERUDESP is gratefully acknowledged. We thank Dr. Josef Zapp, Saarland University, for the NMR and IR determinations, and Birgit Hasper for the technical assistance.
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Gauer, S., Wang, Z., Otten, H. et al. An l-glucitol oxidizing dehydrogenase from Bradyrhizobium japonicum USDA 110 for production of d-sorbose with enzymatic or electrochemical cofactor regeneration. Appl Microbiol Biotechnol 98, 3023–3032 (2014). https://doi.org/10.1007/s00253-013-5180-7
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DOI: https://doi.org/10.1007/s00253-013-5180-7