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Two-day detection of infectious enteric and non-enteric adenoviruses by improved ICC-qPCR

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Abstract

In order to provide a more suitable response to public health concerns, we improved the detection of infectious human adenoviruses in water by optimising the commonly used integrated cell culture–PCR method. Risk evaluation studies seek for rapid detection of infectious adenoviruses, including the enteric types 40 and 41 that are considered as the second most common agents of gastroenteritis in children next to rotaviruses. The here-employed 293A cell line used for infectious status assessment showed its ability to multiply adenoviruses including type 41. Two modifications were moreover applied to the workflow for viral detection. The first occurred at the nucleic acid extraction step performed directly on all infected cells, while the second was the application of real-time quantitative PCR as detection tool. All adaptations led to a 3-day reduction of the response delay and an improved sensitivity especially for the enteric adenoviral types. The infectious status of laboratory strain types 2 and 41 was demonstrated by a more than 2-log10 increase in genome quantity. These conclusions were confirmed and reinforced by the analysis of water samples applying the improved assay. Naturally occurring infectious adenoviruses were detected in wastewater and river water, within 2 days. Types belonging to the species human adenoviruses C and type 31 were observed, but the most frequently identified type was 41 (71 % of identified sequences, n = 34). This highlights the usefulness of our method for a wide range of types, and especially for the most prevalent and public health-relevant enteric adenoviruses.

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Acknowledgements

The present work was carried out in the framework of the PATHOS project (contract number C08/SR/08), supported by the National Research Fund, Luxembourg. This work was also supported by the LorLux network (French name, RésEAU LorLux) and the Moselle Workshop Zone (French abbreviation, ZAM). The authors would like to thank Cécile Walczak for her precious and excellent technical assistance, and also Dr L. Hoffmann for final reading of the manuscript. The LCPME also wishes to thank the Institut Carnot Énergie et Environnement en Lorraine (ICÉEL) for financial support (1.9 Action 009-OTELo).

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Authors and Affiliations

  1. Centre de Recherche Public-Gabriel Lippmann, Department of Environment and Agro-biotechnologies (EVA), 41 rue du Brill, 4422, Belvaux, Luxembourg

    Leslie Ogorzaly, Henry-Michel Cauchie & Christian Penny

  2. Université de Lorraine and CNRS, LCPME (Laboratoire de Chimie Physique et Microbiologie pour l’Environnement), UMR 7564, 54000, Nancy, France

    Aline Perrin, Christophe Gantzer & Isabelle Bertrand

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  1. Leslie Ogorzaly
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  2. Henry-Michel Cauchie
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  3. Christian Penny
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  4. Aline Perrin
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  5. Christophe Gantzer
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  6. Isabelle Bertrand
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Correspondence to Leslie Ogorzaly.

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Ogorzaly, L., Cauchie, HM., Penny, C. et al. Two-day detection of infectious enteric and non-enteric adenoviruses by improved ICC-qPCR. Appl Microbiol Biotechnol 97, 4159–4166 (2013). https://doi.org/10.1007/s00253-013-4782-4

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  • DOI: https://doi.org/10.1007/s00253-013-4782-4

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