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Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

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Abstract

Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.

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Acknowledgements

The research was supported by Academy of Finland: Enzymatic cross-linking of food proteins: impact of food protein folding on the mode of action of cross-linking enzymes (No. 110965), EU: High performance industrial protein matrices (No. NMP-3-CT-2003-505790) and TEKES (The Finnish Funding Agency for Technology and Innovation): Tailored nanostabilisers for biocomponent interfaces (TAINA, no: VTT-R-06743-08). In addition, Outi Liehunen, Riitta Isoniemi and Päivi Matikainen are thanked for the technical assistance.

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Authors and Affiliations

  1. The Faculty of Biological and Environmental Sciences, University of Helsinki, Viikinkaari 9, P.O. Box 56, Helsinki, 00014, Finland

    Outi Nivala

  2. VTT Technical Research Centre of Finland, P.O. Box 1000, 02044, Vuorimiehentie, Finland

    Outi Nivala, Maija-Liisa Mattinen, Greta Faccio, Johanna Buchert & Kristiina Kruus

  3. Empa, Swiss Federal Laboratories for Materials Science and Technology - Laboratory for Biomaterials, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland

    Greta Faccio

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  1. Outi Nivala
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  2. Maija-Liisa Mattinen
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  3. Greta Faccio
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  4. Johanna Buchert
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  5. Kristiina Kruus
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Correspondence to Maija-Liisa Mattinen.

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Nivala, O., Mattinen, ML., Faccio, G. et al. Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen. Appl Microbiol Biotechnol 97, 9429–9437 (2013). https://doi.org/10.1007/s00253-013-4753-9

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  • DOI: https://doi.org/10.1007/s00253-013-4753-9

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