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Molecular cloning and characterization of a GH11 endoxylanase from Chaetomium globosum, and its use in enzymatic pretreatment of biomass

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Abstract

An endo-1,4-β-xylanase gene, xylcg, was cloned from Chaetomium globosum and successfully expressed in Escherichia coli. The complete gene of 675 bp was amplified, cloned into the pET 28(a) vector, and expressed. The optimal conditions for the highest activity of the purified recombinant XylCg were observed at a temperature of 40 °C and pH of 5.5. Using oat-spelt xylan, the determined K m, V max, and k cat/K m values were 0.243 mg ml−1, 4,530 U mg−1 protein, and 7,640 ml s−1 mg−1, respectively. A homology model and sequence analysis of XylCg, along with the biochemical properties, confirmed that XylCg belongs to the GH11 family. Rice straw pretreated with XylCg showed 30 % higher conversion yield than the rice straw pretreated with a commercial xylanase. Although xylanases have been characterized from fungal and bacterial sources, C. globosum XylCg is distinguished from other xylanases by its high catalytic efficiency and its effectiveness in the pretreatment of lignocellulosic biomass.

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Acknowledgments

This research was supported by the Converging Research Center Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2011-50210). This subject was supported by Korea Ministry of Environment as GAIA Project (G112-00055-0023-0). This work was also supported by 2012 KU Brain Pool fellowship of Konkuk University

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Authors and Affiliations

  1. Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, South Korea

    Raushan Kumar Singh, Manish Kumar Tiwari, Yun Chan Kang & Jung-Kul Lee

  2. Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 143-701, South Korea

    Manish Kumar Tiwari, Priyadharshini Ramachandran & Jung-Kul Lee

  3. Phygen Research Institute, Daejeon, South Korea

    Dongwook Kim

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  1. Raushan Kumar Singh
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  2. Manish Kumar Tiwari
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  3. Dongwook Kim
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  4. Yun Chan Kang
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  5. Priyadharshini Ramachandran
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  6. Jung-Kul Lee
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Correspondence to Priyadharshini Ramachandran or Jung-Kul Lee.

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Singh, R.K., Tiwari, M.K., Kim, D. et al. Molecular cloning and characterization of a GH11 endoxylanase from Chaetomium globosum, and its use in enzymatic pretreatment of biomass. Appl Microbiol Biotechnol 97, 7205–7214 (2013). https://doi.org/10.1007/s00253-012-4577-z

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