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Development of a novel recombinant strain Zygosacharomyces rouxii JL2011 for 1,3-propanediol production from glucose

  • Applied genetics and molecular biotechnology
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Abstract

1,3-propanediol (1,3-PDO) is one of the most important industrial chemicals due to its highly desired properties and its wide applications as a key component of the emerging polymer industry. Biotechnology route has been one of the most interesting methods for 1,3-PDO production, whereas, the dha genes were essential to 1,3-PDO biosynthesis. In this study, we cloned and placed the dha cassettes under the control of a glyceraldehyde 3-phosphate dehydrogenase gene promoter pGAP and homologous ZrFPS1 gene promoter pZrfps1; these two promoters were further integrated into the chromosome of Z. rouxii JL2011 to generate recombinant strain JL2011-GZ and JL2011-ZZ, respectively. The results showed that the two strains could produce 1,3-PDO from glucose with a final yield of 6.9 and 10.3 g/l, respectively. The engineered strain JL2011-ZZ showed a 2.3- and 1.5-fold increase in the specific activities and final concentration of 1,3-PDO, respectively, with respect to JL2011-GZ. Batch fermentation with aerobic/micro-aerobic combined strategy of JL2011-ZZ resulted a titer of 17.1 g/l and a yield from glucose of 8.6 %. These results demonstrated that JL2011-ZZ would be a potential strain for 1,3-PDO production from glucose.

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Acknowledgments

This work was supported by the Zhejiang key project of innovative team (2009R500273), Zhejiang S&T project (2010C32063), Zhejiang Province Natural Science Foundation (LQ12C14004, LY12C140046), and National Basic Research Program of China (973 Program; No.2011CB111602, 2012CB114100). We are grateful to Dr. Zhiming Rao for his support in the early stage of this work and Dr. Bin Zhuge for his helpful suggestion.

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang Province, 310018, People’s Republic of China

    Zheng Ma, Yalin Bian, Xuping Shentu & Xiaoping Yu

  2. College of Life Sciences, China Jiliang University, Xueyuan Street, Xiasha Higher Education District, Hangzhou, Zhejiang Province, 310018, People’s Republic of China

    Xiaoping Yu

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  1. Zheng Ma
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  2. Yalin Bian
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  3. Xuping Shentu
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Correspondence to Xiaoping Yu.

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Ma, Z., Bian, Y., Shentu, X. et al. Development of a novel recombinant strain Zygosacharomyces rouxii JL2011 for 1,3-propanediol production from glucose. Appl Microbiol Biotechnol 97, 4055–4064 (2013). https://doi.org/10.1007/s00253-012-4501-6

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  • DOI: https://doi.org/10.1007/s00253-012-4501-6

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