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Photothermal effects of folate-conjugated Au nanorods on HepG2 cells

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Abstract

Photothermal cancer therapy, as a prospective approach for local cancer treatment, is attracting increasing interests. In this paper, gold nanorods were conjugated with folate (folate/AuNRs), and their photothermal effects on hepatocellular carcinoma cell line (HepG2) using MTT assay, flow cytometry, as well as on the cellular morphology, cytoskeleton, cell surface adhesion, and stiffness detected at subcellular level by an atomic force microscope (AFM) were investigated. The results indicated that near-infrared laser-induced hyperthermia of folate/AuNRs could break the cell membrane integrity and homeostasis and then lead to the depolymerization of cytoskeleton and influx of intracellular Ca2+. Thus, folate/AuNRs can be as effective and promising nanomaterials for photothermal therapy of folate receptor bearing tumor.

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Acknowledgment

This work was funded by National Natural Science Foundation of China (30872404, 21071064), 973 program projects (2010CB833603) and Overseas, Hong Kong and Macao Scholars Collaborated Researching Fund (30828028).

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Authors and Affiliations

  1. Department of Chemistry and Institute for Nano-chemistry, Jinan University, Guangzhou, China, 510632

    Hua Jin, Peihui Yang, Jiye Cai, Jinhui Wang & Mei Liu

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  1. Hua Jin
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  2. Peihui Yang
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  3. Jiye Cai
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  4. Jinhui Wang
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  5. Mei Liu
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Correspondence to Peihui Yang.

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Jin, H., Yang, P., Cai, J. et al. Photothermal effects of folate-conjugated Au nanorods on HepG2 cells. Appl Microbiol Biotechnol 94, 1199–1208 (2012). https://doi.org/10.1007/s00253-012-3935-1

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  • DOI: https://doi.org/10.1007/s00253-012-3935-1

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