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Elaboration of antibiofilm materials by chemical grafting of an antimicrobial peptide

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Abstract

A peptide antibiotic, gramicidin A, was covalently bound to cystamine self-assembled monolayers on gold surfaces. Each step of the surface functionalization was characterized by polarization modulation infrared reflection absorption spectroscopy and X-ray photoelectron spectroscopy. The antimicrobial activity of the anchored gramicidin was tested against three Gram-positive bacteria (Listeria ivanovii, Enterococcus faecalis, and Staphylococcus aureus), the Gram-negative bacterium Escherichia coli and the yeast Candida albicans. The results revealed that the adsorbed gramicidin reduced, from 60% for E. coli to 90% for C. albicans, the number of culturable microorganisms attached to the surface. The activity was proven to be persistent overtime, up to 6 months after the first use. The bacteria attached to the functionalized surfaces were permeabilized as shown by confocal microscopy. Taken together, these results indicate a bacteriostatic mode of action of the immobilized peptide. Finally, using green fluorescent protein-expressing bacteria, it was shown that the development of a bacterial biofilm was delayed on peptide-grafted surfaces for at least 24 h.

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Acknowledgments

This work was financially supported by the PNIR Biofilms CNRS and by the French ANR SURFANBAC. We thank P.J. Hill of the University of Nottingham, UK, for providing the pSB2019 plasmid and Dr Z. Piao from Kyushu University, Japan, for providing pPZ1 plasmid.

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Authors and Affiliations

  1. Laboratoire de Chimie et Microbiologie de l′Eau-UMR 6008 CNRS, IBMIG-UFR Sciences fondamentales et Appliquées, Université de Poitiers, 40 avenue du Recteur Pineau, 86022, Poitiers Cedex, France

    Jean-Fabrice Yala, Arnaud Héquet & Jean-Marc Berjeaud

  2. Laboratoire de Réactivité de Surface CNRS, UMR CNRS 7197, Université Pierre et Marie Curie-UPMC Paris 6, 4 place Jussieu, 75252, Paris Cedex 05, France

    Pascal Thebault, Arnaud Héquet, Vincent Humblot & Claire-Marie Pradier

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  1. Jean-Fabrice Yala
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  2. Pascal Thebault
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  4. Vincent Humblot
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  6. Jean-Marc Berjeaud
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Correspondence to Vincent Humblot or Jean-Marc Berjeaud.

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Yala, JF., Thebault, P., Héquet, A. et al. Elaboration of antibiofilm materials by chemical grafting of an antimicrobial peptide. Appl Microbiol Biotechnol 89, 623–634 (2011). https://doi.org/10.1007/s00253-010-2930-7

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  • DOI: https://doi.org/10.1007/s00253-010-2930-7

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